BIOPROSPECÇÃO DE LEVEDURAS COM POTENCIAL PROBIÓTICO ISOLADAS DO FRUTO DO JAMBOLÃO (SYZYGIUM CUMINI)

Authors

DOI:

https://doi.org/10.20873/vol13n3pibic202523

Keywords:

Probiotics; Yeasts; Fermentation; Jambolan; Microbiology.

Abstract

The present study aimed to evaluate the probiotic potential of yeasts isolated from the spontaneous fermentation of jambolan fruit (Syzygium cumini), a tropical species widely distributed in Brazil and recognized for its pharmacological properties. The fruits were collected in Palmas, Tocantins, and subjected to spontaneous fermentation under controlled conditions. Yeasts were isolated on Sabouraud agar and characterized through macro- and micromorphological analyses. The differentiation between Saccharomyces and non-Saccharomyces yeasts was performed using a culture medium containing lysine. Subsequently, the isolates were subjected to in vitro tests to evaluate tolerance to gastric acid, resistance to bile salts, susceptibility to antifungal agents, and antagonistic activity against enteric pathogens. A total of 124 yeasts were obtained, of which 5 were preliminarily classified as Saccharomyces and 119 as non-Saccharomyces. Among these, 44 showed viability under acidic pH conditions, and 12 isolates demonstrated resistance to bile salts and antifungal agents. Three isolates also exhibited antagonistic activity against Shigella sonnei. The results indicate that yeasts isolated from jambolan have potential for probiotic applications and represent a promising source of microorganisms with biotechnological relevance.

 

 

References

AMORIM, J.; PICCOLI, R.; DUARTE, W. Probiotic potential of yeasts isolated from pineapple and their use in the elaboration of potentially functional fermented beverages. Food Research International, v. 107, p. 518–527, 2018.

AUGUSCO, C. A. M. Análise fitoquímica e estudo das atividades antimicrobiana, antioxidante e toxicológica das folhas da Couroupita guianensis (Aubl.). 2022. Dissertação (Mestrado) – Universidade Federal do Tocantins, Palmas, 2022.

ARCHER, A. C.; HALAMI, P. M. Probiotic attributes of Lactobacillus fermentum isolated from human feces and dairy products. Applied Microbiology and Biotechnology, v. 99, n. 19, p. 8113-8123, 2015.

BEVILACQUA, A. et al. Technological and spoiling characteristics of the yeast microflora isolated from table olives. International Journal of Food Science and Technology, v. 44, n. 11, p. 2198-2207, 2009.

CASTAGLIUOLO, I. et al. Saccharomyces boulardii protease inhibits the effects of Clostridium difficile toxins. Infection and immunity, v. 67, n. 1, p. 302-307, 1999.

CHANG, L. S. et al. Characterization of enzyme-liquefied soursop (Annona muricata L.) puree. LWT, v. 94, p. 40–49, 2018.

CHHIKARA, N. et al. Bioactive compounds and pharmacological applications of Syzygium cumini. Food & function, v. 9, n. 12, p. 6096-6115, 2018.

COUTINHO, J. O. P. A. et al. In vitro and in vivo evaluation of the probiotic potential of Antarctic yeasts. Probiotics and Antimicrobial Proteins, v. 13, n. 5, p. 1338–1354, 2021.

DA SILVA, K. A. G. et al. Caracterização microbiológica, físico-química e sensorial do suco de jambolão (Syzygium cumini). Brazilian Journal of Development, v. 7, n. 5, p. 50597-50613, 2021.

DE MIRANDA, N. M. Z. et al. Novel yeasts with potential probiotic characteristics isolated from artisanal cheese. Brazilian Journal of Microbiology, v. 54, n. 2, p. 1021-1033, 2023.

DIOSMA, G. et al. Yeasts from kefir grains: isolation, identification and probiotic characterization. World Journal of Microbiology and Biotechnology, v. 30, n. 1, p. 43-53, 2014.

DORON, S.; SNYDMAN, D. Risk and safety of probiotics. Clinical Infectious Diseases, v. 60, n. suppl_2, p. S129-S134, 2015.

FAO; WHO. Guidelines for the evaluation of probiotics in food. London: FAO/WHO Working Group Report, 2002.

FERREIRA, E. M. S. et al. A safe method to control anthracnose in papaya. Revista Brasileira de Fruticultura, v. 40, p. e-683, 2018.

MATILLA-SANDHOLM, T. et al. Technological challenges for future probiotic foods. International Dairy Journal, v. 12, n. 2-3, p. 173-182, 2002.

NAWAZ, A. S.; NOOR, N. et al. Characterization of lactic acid bacteria isolated from fermented foods for probiotic potential. International Journal of Current Microbiology and Applied Sciences, v. 6, n. 7, p. 3975–3980, 2017.

OLIVEIRA, F. M. N. de; SOUZA, J. A.; GOMEZ-CÁCERES, L. F. Fatores que interferem durante o processo de fermentação natural das fatias de cubiu. Revista Colombiana de Ciencia Animal, v. 9, n. 1, p. 81–88, 2017.

PINHEIRO, P. N.; HORNES, M. O. Caracterização físico-química e sensorial de fermentado de jambolão (Syzygium cumini). Revista Brasileira de Tecnologia Agroindustrial, v. 15, n. 2, 2021.

RIBEIRO, M. C. de O. Caracterização do Pediococcus acidilactici B14 quanto às propriedades probióticas e sua associação com Lactobacillus acidophilus ATCC 4356. 2012. Dissertação – Universidade Federal do Paraná, 2012.

SÁNCHEZ-ORTIZ, A. C. et al. Isolation and characterization of potential probiotic bacteria from Anadara tuberculosa. Latin American Journal of Aquatic Research, v. 43, n. 1, p. 123–136, 2015.

SANTOS, T. C. Identificação e avaliação do potencial probiótico de leveduras isoladas de frutos amazônicos. 2023. 66 f. Dissertação (Mestrado) – Universidade Federal do Tocantins, Palmas, 2023.

SILVA, J. F. M. da et al. Use of probiotics to control aflatoxin production in peanut grains. The Scientific World Journal, v. 2015, n. 1, p. 959138, 2015.

Published

2026-05-13

How to Cite

Durão, G. G. D., Silva, J. F. M., Cavalcante, J. M. C., Barros, V. L. B., & Silva, D. L. da S. (2026). BIOPROSPECÇÃO DE LEVEDURAS COM POTENCIAL PROBIÓTICO ISOLADAS DO FRUTO DO JAMBOLÃO (SYZYGIUM CUMINI). DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 13(3), 303–314. https://doi.org/10.20873/vol13n3pibic202523

Issue

Section

PIBIC 2024-2025

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