Xilanases: principais metodologias e parâmetros cinéticos
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v2n2.santosKeywords:
enzymatic activity, purification techniques, immobilization of enzymes, xylan, thermostabilityAbstract
Endo-1,4-β-xylanases are extracellular enzymes that make the hydrolysis of substrates β 1,4. Xylan is the main representative among the substrates that are susceptible to hydrolytic attack of xylanases. Xylanases have a wide application in different sectors of the economy. The purpose of this study was to review the published literature on the main methodologies used to study these enzymes, as well as gather some kinetic parameters. The reviewed methodologies are centered mainly in the purification process, since that is an essential step for the optimization of its enzymatic activity and therefore industrial application. The highlights are the techniques that use the saline precipitation and refined methodologies as chromatography and its variations, as well as factorial design studies and enzyme immobilization. These methodologies aim respectively, among other things, optimal conditions for enzyme production and recycling.
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