Xilanases: principais metodologias e parâmetros cinéticos

Autores/as

  • Leandro Freire dos Santos Universidade Federal do Paraná
  • Priscila Lumi Ishii
  • Leandro Freire dos Santos Universidad Federal de Paraná - UFPR https://orcid.org/0000-0003-4482-3706
  • Priscila Lumi Ishii Universidad Estatal Paulista Julio de Mesquita Filho - UNESP

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v2n2.santos

Palabras clave:

técnicas de purificação, imobilização de enzimas, xilanas, termoestabilidade, atividade enzimática

Resumen

As endo β 1,4 xilanases são enzimas extracelulares que fazem a hidrólise de substratos que possuem ligações do tipo β 1,4. A xilana é o principal representante dentre os substratos susceptíveis ao ataque hidrolítico das xilanases. Encontra vasta aplicação em diferentes setores da economia. O objetivo do presente trabalho foi revisar a literatura publicada sobre as principais metodologias utilizadas para se trabalhar com estas enzimas, bem como reunir alguns parâmetros cinéticos. As metodologias revisadas estão centralizadas principalmente no processo de purificação, haja vista que é uma etapa essencial para otimização da sua atividade enzimática e conseguinte aplicação industrial. Destacam-se as técnicas que utilizam a precipitação por sais e metodologias refinadas como a cromatografia e suas variações. Tem ganhado destaque, em pesquisas acadêmicas, os estudos de planejamentos fatoriais e imobilização enzimática. Tais metodologias buscam respectivamente, dentre outras coisas, condições otimizadas de produção e reciclagem enzimática.

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Publicado

2011-08-01

Cómo citar

dos Santos, L. F., Ishii, P. L., Santos, L. F. dos, & Ishii, P. L. (2011). Xilanases: principais metodologias e parâmetros cinéticos. Journal of Biotechnology and Biodiversity, 2(2), 7–15. https://doi.org/10.20873/jbb.uft.cemaf.v2n2.santos