Xilanases: principais metodologias e parâmetros cinéticos
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v2n2.santosPalabras clave:
técnicas de purificação, imobilização de enzimas, xilanas, termoestabilidade, atividade enzimáticaResumen
As endo β 1,4 xilanases são enzimas extracelulares que fazem a hidrólise de substratos que possuem ligações do tipo β 1,4. A xilana é o principal representante dentre os substratos susceptíveis ao ataque hidrolítico das xilanases. Encontra vasta aplicação em diferentes setores da economia. O objetivo do presente trabalho foi revisar a literatura publicada sobre as principais metodologias utilizadas para se trabalhar com estas enzimas, bem como reunir alguns parâmetros cinéticos. As metodologias revisadas estão centralizadas principalmente no processo de purificação, haja vista que é uma etapa essencial para otimização da sua atividade enzimática e conseguinte aplicação industrial. Destacam-se as técnicas que utilizam a precipitação por sais e metodologias refinadas como a cromatografia e suas variações. Tem ganhado destaque, em pesquisas acadêmicas, os estudos de planejamentos fatoriais e imobilização enzimática. Tais metodologias buscam respectivamente, dentre outras coisas, condições otimizadas de produção e reciclagem enzimática.
Citas
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