Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens

Authors

  • André Luís Lopes da Silva Universidade Federal do Paraná
  • Yohana de Oliveira
  • Jefferson da Luz Costa
  • Clarissa de Souza Mudry
  • Marcia Procopiuk
  • Gessiel Newton Scheidt
  • Gilvano Ebling Brondani
  • André Luis Lopes da Silva Universidade Federal do Paraná
  • Yohana Oliveira Universidade Federal do Paraná
  • Jefferson da Luz Costa Universidade Federal do Paraná https://orcid.org/0000-0002-8912-8208
  • Clarissa de Souza Mudry Universidade Federal do Paraná
  • Gessiel Newton Scheidt Universidade Federal do Paraná https://orcid.org/0000-0002-4729-3416
  • Gilvano Ebling Brondani http://orcid.org/0000-0001-8640-5719

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v2n1.silva

Keywords:

Eucaliptos, gene GUS, linhagem EHA105

Abstract

The regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation of shoot tips of Eucalyptus saligna via Agrobacterium tumefaciens and to promote the explants selection supposedly transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days before the transformation. Strain EHA105 of A. tumefaciens harboring the plasmid pBI121 was used. The treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured in multiplication medium supplemented with 250 mg.L-1 Cefotaxime® and each sub-culture the kanamycin levels were increased from 50 to 150 mg.L-1. The transient expression of the uidA gene in shoot tips was evaluated after the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence of 100 µM acetosyringone at the co-culture of shoot tips of Eucalyptus saligna promoted higher transient expression of the uidA gene and retards toxic effects caused by kanamycin.

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Published

2011-01-10

How to Cite

da Silva, A. L. L., de Oliveira, Y., Costa, J. da L., Mudry, C. de S., Procopiuk, M., Scheidt, G. N., … Brondani, G. E. (2011). Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, 2(1), 1–6. https://doi.org/10.20873/jbb.uft.cemaf.v2n1.silva

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