Isolation and identification of Lactobacillus sp. and Saccharomyces sp. from spontaneous fermentation of amazonian fruit (pupunha) with probiotic and biotechnological potential for the development of new food products

Authors

  • Juliana Fonseca Moreira Silva Medicina / UFT
  • Wilson Lopes Miranda
  • Geovanka Marcelle Aguiar Leão

DOI:

https://doi.org/10.20873/uftsupl2021-12530

Abstract

 

The Amazon holds enormous native reserves, rich in fruits that have great social and economic importance, among which stands out the Pupunha (Bactris gasipaes). Through biotechnology it is possible to make use of microorganisms with probiotic potential associated with these regional fruits and available in this region. Thus, this work aimed to isolate microbial strains (Lactobacillus sp. and Saccharomyces sp.) with probiotic and biotechnological potential, obtained from spontaneous fermentation of the Pupunha fruit. The fruits, obtained from free markets in Manaus, were submitted to spontaneous fermentation for 15 days. After this period 185, microorganisms were isolated and characterized regarding their morphology, catalase test, gram staining, and some probiotic potential tests such as antagonism against enteropathogens and temperature test. The isolates were grouped into 4 different morphotypes presenting gram + staining, catalase - and coccobacilli micromorphology suggestive of Lactobacillus sp. All showed resistance to high temperatures, and morphotypes I and II presented a greater antagonist potential against pathogenic strains. During the whole process of spontaneous Pupunha fermentation, no isolates of yeasts of the genus Saccharomyces sp. were observed.      

Published

2021-11-03

How to Cite

Silva, J. F. M., Miranda, W. L. ., & Leão, G. M. A. . (2021). Isolation and identification of Lactobacillus sp. and Saccharomyces sp. from spontaneous fermentation of amazonian fruit (pupunha) with probiotic and biotechnological potential for the development of new food products . DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 8(Especial), 14–22. https://doi.org/10.20873/uftsupl2021-12530

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