Studies of antagonistic effect between Lactobacillus sakei on Escherichia coli, Listeria monocytogenes and Staphylococcus aureus

Autores/as

  • Universidade Federal do Tocantins
  • Augustus Caeser Franke Portella Universidade Federal do Tocantins
  • Raimundo Wagner de Souza Aguiar Universidade Federal do Tocantins
  • Jefferson da Luz Costa Universidade Federal do Tocantins
  • André Luís Lopes da Silva Universidade Federal do Paraná
  • Gessiel Newton Scheidt Universidade Federal do Tocantins

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v3n1.portella

Palabras clave:

Antimicrobial activity, complex substrates, surface response

Resumen

The antimicrobial activity of a commercial probiotic culture, Lactobacillus sakei(ATCC 1521) at following
concentrations (30, 42, 60, 78 and 90 μg/ml, 105 - 107UFC/mL), temperatures of interaction (4, 10, 20, 30, 37 °C) and initial pH (3.5, 4.0, 4.5, 5.0 and 5.5) were tested against three foodborne pathogens, Escherichia coli, Listeria monocytogenesand Staphylococcus aureus. The antagonistic effect of the probiotic culture in vitro was performed by liquid microdilution method. The results indicated that the inhibitory substance present on a 24 hours culture broth could be an advantage when keeping the culture dominant during longer fermentations. For the highest lactic acid production, the incubation period of lactic acid bacteria (1.04 % v/v) was on MRS Broth in aerobic conditions, at 37 ºC/24 hours, which gave a minimum pH value of the supernatant (3.5). The data suggest that supernatant can have significant bacteriostatic activity against E. coli, L. monocytogenesand S. aureus, and may provide curedmeats with a degree of protection against this microorganism, particularly if employed with a combination of acid pH, and adequate refrigeration.

Citas

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Publicado

2012-02-15

Cómo citar

Augustus Caeser, Raimundo Wagner de Souza, Jefferson da Luz, André Luís, Gessiel Newton, Portella, A. C. F., … Scheidt, G. N. (2012). Studies of antagonistic effect between Lactobacillus sakei on Escherichia coli, Listeria monocytogenes and Staphylococcus aureus. Journal of Biotechnology and Biodiversity, 3(1), 10–17. https://doi.org/10.20873/jbb.uft.cemaf.v3n1.portella

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