Physiological responses of Aechmea setigera Mart. ex Schult. & Schult. f. (Bromeliaceae) in vitro cultured
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v10n1.silvaPalabras clave:
Micropropagation, stomatal density, chlorophyll, carbohydrates, phenolic compoundsResumen
In vitro propagation of bromeliads is widely used to produce plantlets intended for conservation. This study aims at evaluating physiological responses of Aechmea setigera in vitro cultured under different concentrations of 6-benzylaminopurine (BAP). The experiment was conducted in completely randomized design with five treatments (0, 1.0, 2.0, 4.0 and 8.0 mg L-1 of BAP). After 90 days of in vitro cultured in stationary liquid MS culture medium it were assessed morphological and biochemical parameters. The BAP increment increased stomatal density, reduced plant size and root number, but increased the number of shoots. There was significant effect of BAP dose on fresh and dry mass. High BAP doses (4.0 and 8.0 mg L-1) promoted the reduction of chlorophyll a and total chlorophyll content, but there was no change of chlorophyll b and carotenoids. The concentration of starch and total soluble carbohydrates decreased above 2.0 and 4.0 mg L-1 of BAP, respectively. The total phenolic content decreased with increasing BAP concentrations. Evaluated changes in metabolism elucidate the phenotypic plasticity for in vitro multiplication process in A. setigera.
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Aguiar TV, Santanna-Santos BF, Azevedo AA, Ferreira RS. ANATI QUANTI: Software de análises quantitativas para estudos em anatomia vegetal. Planta Daninha, v.25, n.4, p.649-659, 2007. https://doi.org/10.1590/S0100-83582007000400001
Aliniaeifard S, Asayesh ZM, Driver J, Vahdati K. Stomatal features and desiccation responses of Persian walnut leaf as caused by in vitro stimuli aimed at stomatal closure. Trees, v.34, p.1219-1232, 2020. https://doi.org/10.1007/s00468-020-01992-x
Aloni R. Foliar and axial aspects of vascular differentiation: Hypotheses and evidence. Journal of Plant Growth Regula-tion, v.20, n.1, p.22-34, 2001. https://doi.org/10.1007/s003440010001
Borghezan M, Moraes LKA, Moreira FM, Silva AL. Propa-gação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira. Pesquisa Agropecuária Brasi-leira, v.38, n.7, p.185-194, 2003
Brito EG, Sousa JS, Carvalho WV, Gurgel ESC. Estudo taxonômico das angiospermas epífitas de Belém, Pará, Bra-sil. Boletim do Museu Paraense Emílio Goeldi. Ciências Na-turais, v.14, n.3, p.363-389, 2019. https://doi.org/10.46357/bcnaturais.v14i3.222
Chu EP, Tavares AR, Kanashiro S, Giampaoli P, Yokota ES. Effects of auxins on soluble carbohydrates, starch and solu-ble protein content in Aechmea blanchetiana (Bromeliaceae) cultured in vitro. Scientia Horticulturae, v.125, n.3, p.451-455, 2010. https://doi.org/10.1016/j.scienta.2010.04.021
Cortleven A, Noben JP, Valcke R. Analysis of the photosyn-thetic apparatus in transgenic tobacco plants with altered en-dogenous cytokinin content: a proteomic study. Proteome Science, v.9, n.1, p.1-14, 2011.
Dias MO, Sousa MJ, Alves RC, Ferreira ICFR. Exploring plant tissue culture to improve the production of phenolic compounds: A review. Industrial Crops and Products, v.82, p.9-22, 2016. http://dx.doi.org/10.1016/j.indcrop.2015.12.016
Dobránszki J, Silva JAT. Micropropagation of apple - A review. Biotechnology Advances, v.28, n.4, p.462-488, 2010. https://doi.org/10.1016/j.biotechadv.2010.02.008
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Snith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, v.28, p.350-356, 1956.
Faria DV, Simão MJ, Cipriano R, Werner ET, Soares TCB, Aoyama EM, Lima-Gontijo ABP. In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. Ex Schult. f. (Bromeliaceae) from leaf explants. In Vitro Cellular & Developmental Biology- Plant, v.54, p.530-536, 2018. https://doi.org/10.1007/s11627-018-9907-0
Galvanese MS, Tavares AR, Aguiar FFA, Kanashiro S, Chu EP, Stancato GC, Harder ICF. Efeito de ANA, 6-BA e Agar na propagação in vitro de Aechmea Blanchetiana (Ba-ker) L.B. Smith, bromélia nativa da Mata Atlântica. Ceres, v.54, n.311, p.63-67, 2007.
Guerra MP, Dal Vesco LL. Strategies for the micropropaga-tion of bromeliads. In: Jain, S.M.; Ochatt, S.J. (ed.). Proto-cols for in Vitro Propagation of Ornamental Plants, Meth-ods in Molecular Biology, v.589, p.47-66, 2010. https://doi.org/10.1007/978-1-60327-114-1_6
Hazarika BN. Morpho-physiological disorders in vitro culture of plants. Scientia Horticulturae, v.108, n.2, p.105-120, 2006. https://doi.org/10.1016/j.scienta.2006.01.038
Hiscox JD, Israelstam GF. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, v.57, n.12, p.1332-1334, 1979. https://doi.org/10.1139/b79-163
Koch AK, Monteiro RF, Ilkiu-Borges AL. Checklist de Bro-meliaceae da região da Volta Grande do Xingu, Pará, Brasil. Rodriguésia, v.66, n.2, p.455-464, 2015. https://doi.org/10.1590/2175-7860201566213
Kurita FMK, Tamaki V. In vitro growth of the bromeliad Alcantarea imperialis (Carrière) Harms with different con-centrations of nitrogen. Acta Scientiarum. Biological Scien-ces, v.36, n.3, p.279-285, 2014. https://doi.org/10.4025/actascibiolsci.v36i3.22933
Leão JRA, Vasconcelos JM, Beltrão RT, Raposo A, Fermino Jr PCP. Micropropagação de Aechmea setigera Mart. ex Schult. e Schult. f.: uma bromélia endêmica da Amazônia Ocidental. Biota Amazônia, v.4, n.3, p.117-123, 2014. http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v4n2p117-123
Malá J, Máchová R, Cvrcková H, Karady M, Novák O, Miku-lik J, Dostál J, Strnad M, Dolezal K. The role of cytokinins during micropropagation of wych elm. Biologia Plantarum, v.57, p.174-178, 2013. https://doi.org/10.1007/s10535-012-0252-6
Martins JPR, Pasqual M, Martins AD, Rineira SF. Effects of salts and sucrose concentration on in vitro propagation of Billbergia zebrine (Herbert) Lindley (Bromeliaceae). Aus-tralian Journal of Crop Science, v.9, n.1, p.85-91, 2015.
McCready RM, Guggolz J, Silviera V, Owens HS. Determi-nation of starch and amylose in vegetables. Analytical Chemistry, v.22, n.9. p.1156–1158, 1950. https://doi.org/10.1021/ac60045a016
Missouri Botanical Garden, 2021. Tropicos.org. Disponível em: http://www.tropicos.org. Acesso em: 20 outubro 2021.
Morel GM, Wetmore RH. Tissue culture of monocotyledons. American Journal of Botany, v.38, n.2, p.138-140, 1951.
Murashige T, Skoog FA. Revised medium for rapid growth and bio assays with tabacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.
Pospíšilová J, Tichá I, Kadleček P, Haisel D, Plzáková Š. Acclimatization of micropropagated plants to ex vitro condi-tions. Biologia Plantarum, v.42, p.481-497, 1999. https://doi.org/10.1023/A:1002688208758
Quaresma AC, Jardim MA. Diversidade de bromeliáceas epífitas na Área de Proteção Ambiental Ilha do Combu, Be-lém, Pará, Brasil. Acta Botanica Brasilica, v.26, n.2, p.290-294, 2012. https://doi.org/10.1590/S0102-33062012000200006
Rosa WS, Martins JPR, Rodrigues ES, Rodrigues LCA, Gontijo ABPL, Falqueto AR. Photosynthetic apparatus per-formance in function of the cytokinins used during the in vitro multiplication of Aechmea blanchetiana (Brome-liaecae). Plant Cell, Tissue and Organ Culture, v.133, p.339-350, 2018. https://doi.org/10.1007/s11240-018-1385-x
Santa-Rosa S, Souza FVD, Vidal ÁM, Ledo CAS, Santana JRF. Micropropagation of the ornamental vulnerable brome-liads Aechmea blanchetiana and Aechmea distichantha. Horticultura Brasileira, v.31, p.112-118, 2013. https://doi.org/10.1590/S0102-05362013000100018
Silva LL, Costa JL, Alcantara GB, Carvalho DC, SchuckMR, Biasi LA, Scheidt GN, Soccol CR. Micropropagation of Nidularium innocentii Lem. and Nidularium procerum Lindm. (Bromeliaceae). Pakistan Journal of Botany, v.44, n.3, p.1095-1101, 2012.
Silva SSS, Souza EH, Nepomuceno CF, Souza FVD, Costa MAPC. Micropropagation and in vitro conservation of Al-cantarea nahoumii (Bromeliaceae), an endemic and endan-gered species of the Brazilian Atlantic Forest. Acta Scienti-arum. Biological Sciences, v. 42, e52940, 2020. https://doi.org/10.4025/actascibiolsci.v42i1.52940
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, v.16, p.144-158, 1965.
Tavares AR, Giampaoli P, Machado J, Kanashiro S, Chu EP, Pita PB. Structural analysis of the foliar epidermis during acclimatization of Aechmea blanchetiana (Bromeliaceae) in vitro cultured. Horticultura Brasileira, v.33, n.1, p.45-50, 2015. https://doi.org/10.1590/S0102-053620150000100008
Vasconcelos JM, Leão JRA, Raposo A, Fermino Junior PCP. Sistemas de cultivo in vitro e aclimatização de Aechmea seti-gera Mart. ex Schult. & Schult. f. (Bromeliaceae). Scientia Agraria Paranaensis, v.14, n.4, p.240-246, 2015.
Veselova SV, Farhutdinov RG, Veselov SY, Kudoyarova GR, Veselov DS, Hartung W. The effect of root cooling on hormone content, leaf conductance and root hydraulic con-ductivity of durum wheat seedlings (Triticum durum L.). Journal of Plant Physiology, v.162, n.1, p.21-26, 2005. https://doi.org/10.1016/j.jplph.2004.06.001
Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, v.144, n.3, p.307-313, 1994. https://doi.org/10.1016/S0176-1617(11)81192-2
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