Evaluation of native isolates of Trichoderma and inhibitory effect on Sclerotinia sclerotiorum
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v13n4.20953Keywords:
white mold, soil fungi, biological control, microbiotaAbstract
The fungus Sclerotinia sclerotiorum is the causal agent of white mold, a disease of great importance in many species of cultivated plants. It is a pathogen that is difficult to control and forms resistance structures (sclerotia), capable of remaining in the area for many years. Seeking more sustainable methods of controlling this disease, biological control stands out, such as, for example, with the antagonistic fungus Trichoderma. The present work aimed to evaluate the presence and inhibitory effect of Trichoderma isolates native to the region of Laranjeiras do Sul/PR, on Sclerotinia Sclerotiorum. Different bioassays were carried out to evaluate the effect of the isolates on S. sclerotiorum in vitro and also the protective effect in vivo on lettuce plants. Three Trichoderma isolates were identified that promoted significant inhibition of the mycelial growth of S. sclerotiorum, as well as the formation of sclerotia in vitro. The isolates also promoted a reduction in the severity of white mold on lettuce plants. The results obtained demonstrate the natural occurrence of Trichoderma isolates in Laranjeiras do Sul with significant effect on the control of S. sclerotiorum.
References
Abdullah, M.T.; Ali, N.Y.; Suleman, P. Biological control of Sclerotinia Sclerotiorum (Lib.) de Bary with Trichoderma harzianum and Bacillus amyloliquefaciens. Crop Protection, Oxford, v. 27, p. 1354-1359, 2008.
Agrios, G.N. Plant Pathology. New York: Academic Press. 2005. 922p.
Bell, D. K.; Wells, H. D.; Markham, C. R. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, v.72, p.379-382, 1982.
Bernardo, J. T.; Aguilera, J.G.; Silva, R.B.; Vian, R.; Niella, G.R.; Ulhoa, C.J.; Medeiros, I.R.E. Isolamento on farm de Trichoderma: uma ferramenta no controle de doenças de solo para os agricultores no Brasil. Revista Eletrônica Cien-tífica da UERGS, v. 5, n. 03, p. 263-270, 2019.
Brito, F. S.; Miller, P. R. M.; Stadnik, M. Presença de Tricho-derma spp. em composto e suas características para o con-trole de fitopatógenos. Revista Brasileira de Agroecologia, Porto Alegre, v. 5, n. 1, p. 43-53, 2010.
Campbell, C. L.; Madden, L. V. Introduction to plant disease epidemiology. New York: John Wiley & Sons, 1990. 532p.
Chagas Junior, A.F.C.; Chagas, L.F.B.; Santos, G.R.; Mar-tins, A.L.L.; Carvalho Filho, M.R.; Miller, L.O. Ação de Trichoderma spp. no controle de Fusarium sp., Rhizoctonia solani e Sclerotium rolfsii. Agri-environmental sciences, v. 4, n. 2, p. 9-15, 2018.
Cubilla-Ríos, A.A.; Mendoza, D.D.R.D.; Romero-Rodrígues, M.C.; Flores-Giubi, M.E.; Barúa-Chamorro, J.E. Antibiosis de proteínas y metabolitos en especies de Trichoderma con-tra aislamientos paraguayos de Macrophomina phaseolina. Agronomía Mesoamericana, v. 30, n. 1, p.63-77, 2019. http://dx.doi.org/10.15517/am.v30i1.34423.
Ethur, L. Z. Dinâmica populacional e ação de Trichoderma no controle de fusariose em mudas de tomateiro e pepineiro. 2006. 154 f. Tese (Doutorado em Agronomia: Área de Con-centração Fitopatologia) – Universidade Federal de Santa Maria, Santa Maria, 2006.
Ferreira, D. F. SISVAR: A computer analysis system to fixed effects split plot type designs. Revista Brasileira de Biome-tria, v.37, n.1, p.529-535, 2019. DOI: .
Fipke, G.M.; De Bastos Pazini, J.; Ethur, L. Z. Antagonismo de isolados de Trichoderma spp. ao Sclerotinia sclerotio-rum em diferentes temperaturas. Magistra, v. 27, n. 1, p. 23-32, 2015.
Han, V.C.; Michael, P.J.; Swift, B.; Bennett, S.J. Biological control of Sclerotinia sclerotiorum: Modes of action of bio-control agents, soil organic amendments, and soil microbi-ome manipulation. Biological Control, v.186(April), 105346, 2023.
https://doi.org/10.1016/j.biocontrol.2023.105346.
Harman, G.E. Myths and dogmas of biocontrol. Changes in perceptions derived from research on Trichoderma harzi-anumT-22. Plant Disease, 84, 4: 376–393, 2000.
Isaias, C.O.; Martins, I.; Silva, J.B.T.; Silva, J.P.; Mello, S.C.M. Ação antagônica e de metabólitos bioativos de Tri-choderma spp. contra os patógenos Sclerotium rolfsii e Ver-ticillium dahliae. Summa Phytopathologica, v. 40, n. 1, p. 34-41, 2014.
Jantsch, F.T.; Silva, V.N.; Mello, E.S.; Preor, A.V. Efeito de diferentes formas de aplicação de Trichoderma na produção de mudas de beterraba. Journal of Biotechnology and Biodi-versity, v.11, n.1, p.135-141, 2023. https://doi.org/10.20873/jbb.uft.cemaf.v11n4.16981
Jiménez-Bremont, J.F.; González-Pérez, E.; Ortega-Amaro, M.A.; Madrigal-Ortiz, S.; Duque-Ortiz, A.; Mendoza-Mendoza, A. Volatile organic compounds emitted by Trichoderma: Small molecules with biotechnological poten-tial. Scientia Horticulturae, v. 325, n.November, 2024.
Krause-Sakate, R; Pavan, M.A.; Moura, M.F.; Kurozawa, C. Doenças da alface. In: Amorim, L. (Eds.). Manual de Fito-patologia: doenças das plantas cultivadas. São Paulo: Ceres, 2016. v. 2, p. 33-39.
Li, X.; Liao, Q.; Zeng, S.; Wang, Y.; Liu, J. The use of Trichoderma species for the biocontrol of postharvest fun-gal decay in fruits and vegetables: Challenges and opportu-nities. Postharvest Biology and Technology, v.219, 113236, 2025.
https://doi.org/10.1016/j.postharvbio.2024.113236
Lilly, G.V.; Barnett, H. L. Physiology of the fungi. New York: McGraw-Hill Book, 1951. 464 p. 1951.
Lopes, F.A.C.; Steindorf, A.F.; Geraldine, A.M.; Brandão, R.S.; Monteiro, V.N.; Lobo Júnior, M.; Coelho, A.S.G.; Ulhoa, C.J.; Silva, R.N. Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Scle-rotinia sclerotiorum. Fungal Biology, Oxford, v. 116, n. 7, p. 815-824, 2012.
Machado, D. F. M.; Parzianello, F.R., Silva, A.C.F.; Antoni-olli, Z.I. Trichoderma no Brasil: o fungo e o bioagente. Re-vista de Ciências Agrárias, v. 35, n. 1, p. 274-288, 2012.
Malik, M.A.; Ahmad, N.; Bhat, M.Y. 2024. The green shield: Trichoderma’s role in sustainable agriculture against soil-borne fungal threats. Current Research in Microbial Scienc-es, v.7, n.(November), 2024. https://doi.org/10.1016/j.crmicr.2024.100313.
Portolan, I.B., Pietrobelli, S.R., Moura, G.S., Fernandes, A.P., Bonome, L.T.S., Franzener, F. Action of Trichodermil 1306 in the control of Fusarium oxysporum f. sp. lycopersici in tomato crops. Arquivos do Instituto Biológico, v.87, e0472019, p.1-7. 2020. .
Rabeendran, N.; Jones, E. E.; Moot, D. J.; Stewart, A. Bio-control of Sclerotinia lettuce drop by Coniothyrium minitans and Trichoderma hamatum. Biological Control, v. 39, n. 3, p. 352-362, 2006.
Yassin, M.T.; Mostafa, A.A.F.; Al-Askar, A.A.; Sayed, S.R.M.; Rady, A.M. Antagonistic activity of Trichoderma harzianum and Trichoderma viride strains against some fusarial pathogens causing stalk rot disease of maize, in vitro. Journal of King Saud University - Science, v.33, n.3, 101363, 2021. DOI: https://doi.org/10.1016/j.jksus.2021.101363
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