Remoção de nutrientes de vinhaça por Klebsormidium flaccidum e produ-ção de biomassa com potencial econômico

Ronald Tarazona Delgado; Mayara dos Santos Guarieiro; Frederico Pacheco Militão; Valéria de Oliveira Fernandes

doi:10.20873/jbb.uft.cemaf.v13n1.18757

Authors

Ronald Tarazona Delgado Universidade Federal do Rio Grande https://orcid.org/0000-0003-1366-6269
Mayara dos Santos Guarieiro Federal University of Espírito Santo https://orcid.org/0000-0002-7972-5594
Frederico Pacheco Militão Universidade Federal do Espírito Santo https://orcid.org/0000-0002-9507-8008
Valéria de Oliveira Fernandes Federal University of Espírito Santo https://orcid.org/0000-0002-1968-3927

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v13n1.18757

Keywords:

effluent, microalgae, remediation, sustainability

Abstract

Ethanol is a sustainable alternative for mobility due to low carbon emissions. In Brazil, sugarcane ethanol production generates a large amount of vinasse, and its primary use in fertirrigation causes negative environmental impacts. This study aims to cultivate the microalga Klebsormidium flaccidum using vinasse as a source of nutrients while simultaneously producing biomass of economic value. Three treatments with concentrations of 10%, 20%, and 30% of diluted vinasse were tested, designated as T1, T2, and T3, respectively. A culture using Bold Basal Medium was considered as control. Although the treatments resulted in lower cell densities compared to the control, there was an increase in dry mass (DM) due to nutrient availability. Photosynthetic pigments (chlorophyll a and carotenoids) were lower in the treatments, but proteins (T2 and T3: 8.72 ± 0.11% DM) increased with the addition of vinasse. Carbohydrates were more abundant in the lower concentration of vinasse (T1: 66.39% DM), while the highest lipid content among the treatments was recorded in T1 (5.74% DM). Among the fatty acids, the presence of myristic acid (T3: 164.4 µg g^-1 DM) and palmitic acid (T2: 176.4 µg g^-1 DM) was highlighted. K. flaccidum demonstrated high efficiency in removing total nitrogen and NH₄⁺ (> 90%) in treatments T2 and T3. The highest removals of total phosphorus, its ionic form PO₄^-3, and potassium also occurred in these treatments. Our results highlight the potential of K. flaccidum in nutrient removal from agricultural wastewater, promoting sustainable economy.

Key-words: algae, effluent, remediation, sustainability

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