Induction of phytoalexins by preparations of yeasts, Trichoderma and Cymbopogon citratus Stapf. essential oil
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v7n3.souzaKeywords:
resistance, gliceolin, deoxyanthocyaninsAbstract
Phytoalexins may be induced by biotic and abiotic agents known as elicitors. In this work, you can evaluate the potential of the essential oil of Cymbopogon citratus Stapf., From the fungus Trichoderma sp. and yeast for phytoalexin induction in sorghum (Sorghum bicolor (L.) Moench) and soybean cotyledons (Glycine max L.) mesocotyls. As yeast Sacharomyces cerevisiae, Sacharomyces boulardii and fungus Trichoderma sp. were tested on samples 0.5; 5; 25; 50; 75 and 100%. Cymbopogon citratus Stapf oil. at 625, 1250, 2500, 5000 and 7500 µg / mL configurations. The trade product accorda® was used as a positive control for soybean testing and Biozyme® for sorghum. Sterile distilled water was tested negative for testing on cultures as well as cultures. As two resistance induction methodologies in different sera are not as follows: in the first the mesocotyls were excised 0.5 cm above the listener node and application tubes, we applied 1 mL of the sample to be tested. The second time the seedlings are sprayed 2 mL of the samples are tested in different sizes. All evaluated substances promote or accumulate phytoalexins in soy and sorghum. Oil treatment was the most efficient, however, in the case of sorghum, as high oil temperatures caused inhibition of seedling growth. The second sorghum resistance induction methodology yields the best results of the first one due to the large number of phytoalexins and the possibility of observing the seedling reaction to each treatment.
References
AMRI, I.; HAMROUNI, L.; HANANA, M.; JAMOUSSI, B.; LEBDI, K. Essential oils as biological alternatives to protect date palm (Phoenix dactylifera L.) against Ectomye-lois ceratoniae Zeller (Lepidoptera: Pyralidae). Chilean jour-nal of agricultural research, 74(3), 273-279, 2014. http://dx.doi.org/10.4067/S0718-58392014000300004
BAZARGANI, M.; ROHLOFF, J. (2016). Antibiofilm activi-ty of essential oils and plant extracts against Staphylococ-cusaureus and Escherichia coli biofilms. Food Control, 61, 156-164. https://doi.org/10.1016/j.foodcont.2015.09.036
BOMFIM, M.P.; SÃO JOSÉ, A.R.; REBOUÇAS, T.N.H.; de ALMEIDA, S.S.; SOUZA, I.V.B.; DIAS, N.O. (2010). Avaliação antagônica in vitro e in vivo de Trichoderma spp. a Rhizopus stolonifer em maracujazeiro amarelo Antagonic effect in vitro and in vivo of Trichoderma spp. to Rhizopus stolonifer in yellow passion fruit. Summa Phytopathologica, 36(1), 61-67. https://doi.org/10.1590/S0100-54052010000100011
BONALDO, S. Efeito de Saccharomyces cerevisiae na síntese de fitoalexinas em sorgo, na germinação e formação de apressórios por fungos fitopatogênicos e na proteção de pe-pino a Colletotrichum lagenarium e sorgo a Colletotrichum sublineolum. 2005. 166f. Tese de Doutorado (Agronomia) - Universidade de São Paulo, Piracicaba, 2005.
BORAH, A.; PAW, M.; GOGOI, R.; LOYING, R.; SAR-MA, N.; MUNDA, S.; LAL, M. Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East In-dia. Industrial Crops and Products, 129, 448-454, 2019. https://doi.org/10.1016/j.indcrop.2018.12.035
COSTA, G.; FERREIRA, J.P.; VITORINO, C.; PINA, M.E.; SOUSA, J.J.; FIGUEIREDO, I.V.; BATISTA, M.T. Polyphenols from Cymbopogon citratus leaves astropicais anti-inflammatory agents. Journal of ethnopharmacology, 178, 222-228, 2016. https://doi.org/10.1016/j.jep.2015.12.016
De LIMA GUIMARÃES, L.G.; das GRAÇAS, C.M.; SOU-ZA, P.E., de ANDRADE, J.; VIEIRAS, S. Atividades anti-oxidante e fungitóxica do óleo essencial de capim-limão e do citral. Revista Ciência Agronômica, 42(2), 464-472, 2011.
De SOUZA FERREIRA, T.P.; MOURÃO, D.D.S.C.; dos SANTOS, G.R.; de LIMA GUIMARÃES, L.G.; PIRES E.C. F.; SANTOS, W.F.; de SOUZA AGUIAR, R.W. Fungistatic activity of essential oil of Lippia sidoides Cham. against Curvularia lunata. African Journal of Agricultural Research, 13(14), 704-713, 2018. doi: 10.5897/AJAR2018.12977.
De SOUZA, M.F.C.; PENTEADO, A.L., de SOUZA, D.R.C.; de QUEIROZ, S.C.D.N. Atividade antimicrobiana “in vitro” de óleos essenciais contra patógenos de peixes/" In vitro" antimicrobian activity of essential oils against fish pathogens. Brazilian Journal of Development, 5(10), 17911-17921, 2019.
DIAS, L.P.; LOPES, A.S.; PINTO, C.E.M.; MOURA, H.F.N.; NASCIMENTO, V.L.V. Fungitoxidade do óleo essencial do capim-limão (Cymbopogon citratus) e da ca-momila (Matricaria recutita) sobre o fitopatógeno Clados-porium cladosporioides. Instituto Federal de Educação, Ci-ência e Tecnologia do Piauí. V CONNEPI-2010. 2010.
DORMAN, H.J.D.; DEANS, S.G. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88(2), 308-316, 2000. https://doi.org/10.1046/j.1365-2672.2000.00969.x
EJIKE, C.E.; GONG, M.; UDENIGWE, C.C. Phytoalexins from the Poaceae: Biosynthesis, function and prospects in food preservation. Food research international, 52(1), 167-177, 2013. https://doi.org/10.1016/j.foodres.2013.03.012
FADINI, M.A.; LEMOS, W.P.; PALLINI, A.; VENZON, M.; MOURÃO, S.A. Herbivoria de Tetranychus urticae Koch (Acari: Tetranychidae) induz defesa direta em moran-gueiro?. Embrapa Amazônia Oriental-Artigo em periódico indexado (ALICE). 2004.
FIRA, D.; DIMKIĆ, I.; BERIĆ, T.; LOZO, J.; STANKOVIĆ, S. Biological control of plant pathogens by Bacillus speci-es. Journal of biotechnology, 285, 44-55, 2018. https://doi.org/10.1016/j.jbiotec.2018.07.044
GUIMARÃES LGDL, CardosoMDG, Zacaroni LM, Lima RK, PimentelFA, Morais AR. Influência da luz e da tempe-ratura sobre a oxidação do óleo essencial de capim-limão (Cymbopogon citratus (DC) Stapf). Quim Nova, 31(6), 1476-1480, 2008.
HUSSEIN, M. M.; ABO-ELYOUSR, K. A.; HASSAN, M. A.; HASHEM, M.; HASSAN, E. A.; ALAMRI, S. A. In-duction of defense mechanisms involved in disease re-sistance of onion blight disease caused by Botrytis al-lii. Egyptian Journal of Biological Pest Control, 28(1), 80, 2018. https://doi.org/10.1186/s41938-018-0085-5
KHALEDI, N.; TAHERI, P.; TARIGHI, S. Antifungal activi-ty of various essential oils against Rhizoctonia solani and M acrophomina phaseolina as maiores bean pathogens. Journal of applied microbiology, 118(3), 704-717, 2015. https://doi.org/10.1111/jam.12730
KHALEDI, N.; TAHERI, P. Biocontrol mechanisms of Trichoderma harzianum against soybean charcoal rot caused by Macrophomina phaseolina. Journal of Plant Protection Research, 56(1), 21-31, 2016. https://doi.org/10.1515/jppr-2016-0004
KUMAR, A.; SHUKLA, R.; SINGH, P.; PRASAD, C.S.; DUBEY, N.K. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fun-gal infestation of food commodities. Innovative food science & emerging technologies, 9(4), 575-580, 2008. https://doi.org/10.1016/j.ifset.2007.12.005
MEINERZ, C.C.; FORMIGHIERI, A.P.; SCHWAN-ESTRADAK, R.F.; DIETERIC, H.C.; FRANZENER, G.; STANGARLIN, J.R. Atividade elicitora de fitoalexinas em sorgo e soja por derivados de avenca (Adiantum capillus-veneris L.). Rev. Bras. Plantas Med, 10(2), 26-31, 2008.
MOTOYAMA, M.M.; SCHWAN-ESTRADA, K.R.F.; STANGARLIN, J.R.; FIORI-TUTIDA, A.C.G.; SCAPIM, C. A. Indução de fitoalexinas em soja e em sorgo e efeito fungitóxico de extratos cítricos sobre Colletotrichum lage-narium e Fusarium semitectum. Acta Scientiarum. Agro-nomy, 25(2), 491-496, 2003. http://dx.doi.org/10.4025/actasciagron.v25i2.2062
NICHOLSON, R.L.; JAMIL, F.F.; SNYDER, B.A.; LUE, W.L.; HIPSKIND, J. Phytoalexin synthesis in the juvenile sorghum leaf. Physiological and Molecular Plant Patho-logy, 33(2), 271-278, 1988. https://doi.org/10.1016/0885-5765(88)90027-6
PEDRAS, M.S.C.; AHIAHONU, P.W. Metabolism and detoxification of phytoalexins and analogs by phytopatho-genic fungi. Phytochemistry, 66(4), 391-411, 2005. https://doi.org/10.1016/j.phytochem.2004.12.032
PETERS, R.J. Uncovering the complex metabolic network underlying diterpenoid phytoalexin biosynthesis in rice and other cereal crop plants. Phytochemistry, 67(21), 2307-2317, 2006. https://doi.org/10.1016/j.phytochem.2006.08.009
SILVA, D.C.; ARRIGONI-BLANK, F.M.; BACCI, L.; BLANK, A.F.; FARO, R.R.N.; PINTO, J.A.O.; PEREI-RA, K.L.G. Toxicidade e alterações comportamentais de óleos essenciais de genótipos de Eplingiellafruticosa e seus principais compostos para Acromyrmex balzani. Crop Pro-tection, 116, 181-187, 2019. https://doi.org/10.1016/j.cropro.2018.11.002
STANGARLIN, J.R.; SCHULZ, D.G.; FRANZENER, G.; ASSI, L.; SCHWAN-ESTRADA, K.R.F.; KUHN, O.J. Indução de fitoalexinas em soja e sorgo por preparações de Saccharomyces boulardii. Arquivos do Instituto Biológico, São Paulo, 77(1), 91-98, 2010.
TAMM, L.; THÜRIG, B.; FLIESSBACH, A.; GOLTLIEB, A.E.; KARAVANI, S.; COHEN, Y. Elicitors and soil man-agement to induce resistance against fungal plant disea-ses. NJAS-Wageningen Journal of Life Sciences, 58(3-4), 131-137, 2011. https://doi.org/10.1016/j.njas.2011.01.001
THALER, J.S.; FIDANTSEF, S.S.; DUFFEY; R.M. BOS-TOCK. Trade-offs in plant defense against pathogens and herbivores: a field demonstration of chemical elicitors of in-duced resistance. J. Chem. Ecol. 25: 1597-1609, 1999.
WULFFNA, PASCHOLATISF. Partial characterization of sorghum phytoalexin elicitors isolated from Saccharomyces cerevisiae. Fitopatologia Brasileira, 24(3), 428-435, 1999.
ZANARDONMT, PASCHOLATISF, FIALHO MB. Resis-tência de plântulas de pepineiro a Colletotrichum lagena-rium induzida por frações de extrato de Saccharomyces ce-revisiae. Pesquisa Agropecuária Brasileira, 44(11), 1499-1503, 2010.
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