ENZYMATIC ACTIVITY AND EVALUATION OF THE SENSITIVITY OF BEEF JERKY SPOILAGE HALOARCHAEA TO ORGANIC ACIDS

Authors

DOI:

https://doi.org/10.20873/DEZ_23_08

Abstract

This study aimed to estimate the production of hydrolytic exoenzymes and to evaluate the sensitivity of two spoilage haloarchaea strains isolated from jerked beef in relation to organic acid preservatives (lactic, citric and glucone-delta-lactone). Amylase, cellulase, lipase, gelatinase and caseinase, specific solid media were used with incubation at 37 °C for a period of 15 days and after the Enzymatic Index (EI) was observed and to evaluate the susceptibility of the deteriorating strains to the due acids used the technique of diffuse disk in agar was used (Kirby-Bauer method) with different acids varying their concentrations from 0.5 to 5% diluted in brine to 20% of NaCl. The results of the enzymatic assays show that both strains were negative when producing exoenzymes, except for the V22 strain that showed a good IE (4.5) for gelatinase production. Regarding the sensitivity of the organic acids tested, it was observed that, in general, the use of a concentration from 2,5% can be used to increase the shelf life of the jerky, but that lactic and citric acids presented higher significant values in relation to the inhibition of growth of the strains tested in vitro at different concentrations.

Author Biographies

Eduardo Henrique Santos Guedes, Universidade Federal do Tocantins

Biotecnologia

Albert Lennon Lima Martins, Universidade Estadual do Tocantins

Produção Vegetal; Microbiologia

Lillian França Borges Chagas, Universidade Federal do Tocantins

Produção Vegetal; Microbiologia

Leticia Bezerra de Almeida, Universidade Federal do Tocantins

Agronomia; Microbiologia

Gabriel Soares Nobrega, Universidade Federal do Tocantins

Agronomia; Produção Vegetal

Published

2023-12-28

How to Cite

Chagas Jr, A. F., Guedes, E. H. S., Martins, A. L. L., Chagas, L. F. B., Almeida, L. B. de, & Nobrega, G. S. (2023). ENZYMATIC ACTIVITY AND EVALUATION OF THE SENSITIVITY OF BEEF JERKY SPOILAGE HALOARCHAEA TO ORGANIC ACIDS. DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 10(3). https://doi.org/10.20873/DEZ_23_08

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