Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v4n3.chagasjuniorKeywords:
Vigna unguiculata, nodulation, nitrogen fixationAbstract
Depending on the nutritional characteristics and hardiness, the cowpea has become an important source of protein in North and Northeast regions of Brazil. The cowpea benefits from biological nitrogen fixation (BNF) and may receive part of the nitrogen needed for culture via symbiosis, which reduces production costs. Aiming to contribute to the optimization of the BNF in the culture of cowpea (Vigna unguiculata (L) Walp) in the cerrado of Tocantins, through the effectiveness of populations and diversity of rhizobia obtained in seven areas with and without crops , phenotypic isolation and characterization were performed (pH, time of growth characteristics of the colonies and mucus). We obtained 72 rhizobia and evaluated in a dendrogram showed a great diversity, with the formation of 18 groups and the five large groups with 70% similarity. The study of morphological and physiological characteristics reveals a fairly wide diversity of rhizobia and is usually related to studies at the DNA level.
References
Alves, B.J.R.; Boddey, R.M.; Urquiaga, S. The success of BNF in soybean in Brazil. Plant and Soil, v. 252, p. 1-9, 2003.
Batista, J.S.S.; Hungria, M.; Barcellos, F.G.; Ferreira, M.C.; Mendes, I.C. Variability in Bradyrhizobium japonicum and B. elkanii seven years after introduction of both the exotic microsymbiont and the soybean host in a cerrados soil. Microbial Ecology, v. 53, n. 2, p. 270-284,2007.
Chagas Jr., A.F. Características agronômicas e ecológicas de rizóbios isolados de solos ácidos e de baixa fertilidade da Amazônia. Tese de Doutorado. Universidade Federal do Amazonas - UFAM/ Instituto Nacional de Pesquisas da Amazônia - INPA. 157p, 2007.
Chagas Jr., A.F.; Rahmeir, W.; Fidelis, R.R.; Santos, R.; Chagas, L. F. B. Eficiência agronômica de estirpes de rizóbio inoculadas em feijão-caupi no cerrado, Gurupi, TO. Revista Ciência Agronômica, v. 41, n. 4, p. 709-714, 2010.
Chagas Jr., A.F.; Santos, G. R.; Melo, R. C. L.; Oliveira, A. G.; Vizioli, B.; Chagas, L. F. B.; Costa, J.L. Effect of natural nodutation in the development of leguminous trees on soils of cerrado in Tocantins. Journal of Biotechnology and Biodiversity, v. 3, 1, p. 38-44, 2012.
Chen, W.M.; Faria, S.M.; Straliotto, R.; Pitard, R.M.; Simões-Araújo, J.L.; Chou, J.F.; Chou, Y.J.; Barrios, E.; Prescott, A.R.; Elliott, G.N.; Sprent, J.I.; Young, J.P.W., James, E.K. Proof that Burkholderia strains from effective symbioses with legumes: a study of novel mimosa-nodulation strains from South America. Applied and Environmental Microbiology, v. 71, n. 11, p. 7461-7471, 2005.
Coutinho, H.L.C.; Oliveira, V.M.; Lovato, A.; Maia, A.H.N.; Manfio, G.P. Evaluation of the diversity of rhizobia in Brazilian agricultural soils cultivated with soybeans. Applied Soil Ecology, v. 13, n. 2, p. 159-167, 1999.
Franco, E.; Andrade, C.A.B.; Scapim, C.A.; Freitas, S.L. Resposta do feijoeiro à aplicação de nitrogênio na semeadura e cobertura no sistema de plantio direto. Acta Scientiarum Agronomy, v. 30, n. 3, p. 427-434, 2008.
Freire Filho, F.R.; Lima, J.A.A.; Ribeiro, V.Q. Feijão caupi: avanços tecnológicos. Freire Filho, F.R.; Lima, J.A.A.; Ribeiro, V.Q (eds.). Brasília, DF. Embrapa Informações Tecnológicas. 519p, 2005.
Freitas, A.D.S.; Vieira, C.L.; Santos, C.E.R.S.; Stamford, N.P.; Lyra, M.C.C.P. Caracterização de rizóbios isolados de jacatupé cultivado em solo salino do Estado de Pernambuco, Brasil. Bragantia, v. 66, n. 3, p. 497-504, 2007.
Fuhrmann, J. Symbiotic effectiveness of indigenous soybean Bradyrhizobium as related to serological, morfhological, rhizobitoxine and hydrogenase phenotypes. Applied and Environmental Microbiology, Washington, v. 56, p. 224-229, 1990.
Germano, M.G.; Galli-Terasawa, L.V.; Chueire, L.M.O.; Hungria, M.; Bangel, E.V.; Campo, R.J. 2003. Identificação de estirpes de Bradyrhizobium japonicum / B. elkanii mais eficientes e competitivas para a cultura da soja e avaliação das respostas à reinoculação em áreas com populações estabelecidas distintas de Bradyrhizobium. In: Hoffmann-Campo, C.B.; Saraiva, O.F. (eds). Resultado de Pesquisa da Embrapa Soja – 2002. Microbiologia do Solo.. Londrina, Embrapa Soja. p. 42-59. (Embrapa Soja. Documentos, 216).
Graham, P.H.; Vance, C.P. Nitrogen fixation in perspective: An overview of research and extension needs. Field Crops Research, v. 65, p. 93-106, 2000.
Hameed, S.; Yasmin, S.; Malik, K.A.; Zafar, Y.; Hafeez, F.Y. Rhizobium, Bradyrhizobium and Agrobacterium strain isolated from cultivated legumes. Biology and Fertility of Soils, v. 39, p. 179-185, 2004.
Hara, F.A.S.; Oliveira, L.A. Características fisiológicas e ecológicas de isolados de rizóbios oriundos de solos ácidos e álicos de Presidente Figueiredo, Amazonas. Acta Amazônica, v. 34, n. 3, p. 343-357, 2004.
Hara, F.A.S.; Oliveira, L.A. Características fisiológicas e ecológicas de rizóbios oriundos de solos ácidos de Iranduba, Amazonas. Pesquisa Agropecuária Brasileira, Brasília, v. 40, n. 7, p. 667-672, 2005.
Hungria, M.; Vargas, M.A.T. Environmental factors affecting grain legumes in the tropics, with an emphasis on Brazil. Field Crops Research, v. 65, p. 151-164, 2000.
Hungria, M.; Chueire, L.M.O.; Coca, R.G.; Megías, M. Preliminary characterization of fast growing rhizobial strains isolated from soyabean nodules in Brazil. Soil Biololy and Biochemistry., v. 33, p. 1349-1361, 2001.
KCS: Kovach Computing Services, 2009. Multi- Variate Statistical Package. Virginia, USA. Disponível Online: www.kovcomp.co.uk∕mvsp∕. Acesso em: abril de 2009.
Lima, A.S.; Pereira, J.P.A.R.; Moreira, F.M.S. Diversidade fenotípica e eficiência simbiótica de estirpes de Bradyrhizobium spp. de solos da Amazônia. Pesquisa Agropecuária Brasileira, Brasília, v. 40, n. 11, p. 1095-1104, 2005.
Liu, J.; Wang, E.T.; Chen, W.X. Diverse rhizobia associated with wood legumes Wisteria sinensis, Cercis racemosa and Amorpha fruticosa grown in the temperate zone of the China. Systematic and Applied Microbiology, v. 28, n. 5, p. 465-477, 2005.
Martins, L.M.V.; Neves, M.C.P.; Rumjanek, N.G. Growth characteristics and symbiotic efficiency of rhzobia isolated from cowpea nodules of the north- east region of Brazil. Soil Biology and Biochemistry., v. 29, n.5/6, p. 1005-1010, 1997.
Martins, L.M.V.; Xavier, G.R.; Rangel, F.W.; Ribeiro, J.R.A.; Neves, M.C.P.; Morgado, L.B.; Rumjanek, N.G. Contribution of biological nitrogen fixation to cowpea: a strategy for improving grain yield in the Semi-Arid Region of
Brazil. Biology and Fertility of Soils, v. 38, n. 5, p.:333-339, 2003.
Norris, D. O.; Date, R. A. Legume bacteriology. In: Shan, N. H.; Bryan, W. W. ed. Tropical Pasture Research – Principles and Methods. Hurley: Commonwealt Bureau of Pasture and Field Crops, 1976. p. 134-174. (Bulletin, 51).
Saleena, L.M.; Loganathan, P.; Rangarajan, S.; Nair, S. Genetic diversity and relationship between Bradyrhizobium strains isolated from blackgram and cowpea. Biology and Fertility of Soil, v. 34, n. 4, p. 276-281, 2001.
Santos, C.E.R.S.; Stamford, N.P.; Neves, M.C.; Rumjanek, N.G.; Borges, R.V.; Freitas, A.D.S. Diversidade de rizóbios capazes de nodular leguminosas tropicais. Revista Brasileira de Ciências Agrárias, v. 2, n. 4, p. 249-256, 2007.
Silva, V.N.; Silva, L.E.S.F. & Figueiredo, V.B. Atuação de rizóbio com rizobactéria promotora de crescecimento em plantas na cultura do caupi (vignia unguiculata [L.] Walp). Acta Scientiarum Agronomy, v. 28, n. 3, p. 407-412, 2006.
Somasegaran, P.; Hoben, H. J. 1985. Methods in legume-Rhizobium technology. NifTAL Project and MIRCEN, Hawaii, 365p.
Vieira, C. L. Efeito de estirpes de rizóbio em cultivares de caupi do agreste paraibano. Dissertação (Mestrado) em Microbiologia do solo, Universidade Federal de Pernambuco, Recife. 39p., 2007.
Vincent, J. M. 1970. A manual for practical study of root nodule bacteria. IBP Handbook N. 15. Blackwell Scient. Publ. 140p.
Xavier, G.R.; Martins, L.M.V.; Zilli, J.E.; Peixoto, R.C.; Rumjanek, N.G. 1997. Protocolo operacional cultivo de planta-isca para isolamento de rizóbio a partir de nódul de planta-isca. Seropédica: Embrapa-Agrobiologia, Dezembro, 1997. 7p. (Embrapa-CNPAB. Documentos, 43.
Zhang, W.T.; Yang, J.K.; Yuan, T.Y.; Zhou, J.C. Genetic diversity and phylogeny of indigenous rhizobia from cowpea (Vigna unguiculata (L.) Walp.). Biology and Fertility of Soil, v. 44, n. 1, p. 201-210, 2007.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 - Journal of Biotechnology and Biodiversity
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0 at http://creativecommons.org/licenses/by/4.0/) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Available at The Effect of Open Access, at http://opcit.eprints.org/oacitation-biblio.html).
