Avaliação da toxicidade de estirpes de Bacillus thuringiensis para Aedes aegypti Linneus, (Díptera: Culicidae)
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v2n2.ootaniKeywords:
bio-insecticide, control biological, dengueAbstract
This work was aimed at to select and to identify strain of Bacillus thuringiensis (Bt) obtained of samples of soil in the state of Tocantins that presented toxicity for the mosquito Aedes aegypti. It was obtained isolated of Bt of the samples of obtained soils and with larger number of colonies in soils with high clay tenor and organic matter. The poisonous activity among the isolated ones obtained it varied by 14.7 to 98% of mortality to larvas of 2º stadium of A. aegypti, with prominence for the isolated TO-392. They were accomplished analyzes for SDS-PAGE where there was the presence of a peptides of approximately 80 kDa, wasn´t visualized us too much isolated, the ancestry A-392 had 98% of mortality about larvas of A. aegypt was tested i, in the period of 48 hours, using a concentration of 1 and 2 % ıof the bacterial .broth. It is verified that B. Thuringiensis isolated of the state of Tocantins presents promising results for they can be used in the production of a bioinseticide against the larvas of this mosquito.
References
Araujo, A. P.; Melo-Santos, M. A. V.; Carlos, S. O.; Rios, E. M. M.; Regis, L. (2007), Evolution of an experimental product based on Bacillus thuringiensis sorovar. Israelensis against Aedes aegypti larvae (Diptera: Culicidae). Biological Control, 41, 339-347.
Becker, N. (2002), Sterilization of Bacillus thuringiensis israelensis products by γ radiation. Journal American Mosquito Control Association, 18, 57-62.
Brasil. Ministério da Saúde, (2009), Reunião de avaliação do monitoramento da resistência das populações de Aedes aegypti do Brasil. Ministério da Saúde, Brasília.
Carvalho, M. S. L.; Caldas, E. D.; Degallier, N.; Vilarinhos, P. T. R.; De Souza, L. C. K. R.; Yoshizawa, M. A. C.; Knox, M. B.; Oliveira, C. (2004), Suscetibilidade de larvas de Aedes aegypti ao inseticida temefós no Distrito Federal. Revista de Saúde Pública, 38, 623-629.
Couch, T. L. (2000), Industrial fermentation and formulation of entomopathogenic bacteria. In: Charles, J.-F., Delécluse, A., Nielsen-Leroux, C. (Eds.), Entomopathogenic bacteria: from laboratory to field application. Kluwer Academic Publishers, Dordrecht, 297-314p.
Chang, C.; Shen, W. K.; Wang, T. T.; Lin, Y. H.; Hsu, E. L.; Dai, S. M. (2009), A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti. Insect Biochemistry and Molecular Biology, 39, 272-278.
Cox, J.; Grillet, M. E.; Ramos, O. M.; Amador, M.; Barrera, R. (2007), Habitat segregation of dengue vectors along an urban environmental gradient. American Journal of Tropical Medicine and Hygiene, 76, 820-826.
Espíndola, C. B.; Guedes, R. N.; De Souza, R. C. (2008), Avaliação da Eficácia do Bacillus thuringiensis var. israelensis no Controle de Formas imaturas do Aedes (Stegomyia) aegypti (Linnaeus, 1762) em Ambiente de Laboratório. EntomoBrasilis, 1, 10-13.
Goldberg, L. J. and Margalit, J. (1977), A bacterial spore demonstrating rapid larvicidial activity against Anopheles sergentii, Uranotaenia uguiculata, Culex univitattus, Aedes aegypti and Culex pipiens. Mosquito. News, 37, 355–361.
Glare, T. R. and O’Callaghan, M. (2000), Bacillus thuringiensis: biology, ecology and safety. Chichester: John Wiley, 350p.
Gluber, D. J. (2004), The changing epidemiology of yellow fever and dengue, 1900 to 2003: full circle. Comparative Immunology, Microbiology & Infectious Diseases, 27, 319-330.
Guzman, M. G. and Kouri, G. (2002), Dengue: ans update. Journal of Infectious Diseases, 2, 33-42.
Habib, M. E. M.; Andrade, C. F. S. (1998), Bactérias entompatogênicas In: Alves, S.B. Controle microbiano de insetos. Piracicaba: FEALQ, 12, 383-446p.
Hofte, H. and Whiteley, H. R. (1989), Insecticidal crystal proteins of Bacillus thuringiensis. Microbiology Reviews, 53, 242-255.
Jung, Y. C.; Kim, S. U.; Côte, J. C.; Lecadet, M. M.; Chung, Y. S.; Bok, S. H. (1998), Characterization of a new Bacillus thuringiensis subsp. Higo strain isolated from rice bran in korea. Journal of invertebrate pathology, 71, 95-96.
Kroeger, A.; Horstick, O.; Riedl, C.; Kaiser, A.; Becker, N. (1995), The potential for malaria control with the biological larvicide Bacillus thuringiensis israelensis (Bti) in Peru and Ecuador. Acta Tropical, 60, 47-57.
Lecadet, M. M.; Chaufaux, J.; Ribier, J. E.; Lereclus, D. (1991), Construction of novel Bacillus thuringiensis strains with different insecticidal activities by transduction ande transformation. Applied and Environmental Microbiology, 58, 840-849.
Lima, J. B. P.; Cunha, M. P. Da; R. C.; Silva, Jr. Da; Galardo, A. K. R.; Soares, S. S. Da; Braga, I. A.; Ramos, R. P. & Valle, D. (2003), Resistance of Aedes aegypti to organophosphates in several municipalities in the state of Rio de Janeiro and Espírito Santo, Brazil. American Journal of Tropical Medicine Hygiene, 68, 329-333.
Macoris, M. L. G.; Andrighetti, M. T. M.; Takaku, L.; Glasser, C. M.; Garbeloto, V. C.; Bracco, J. E. (2003), Resistance of Aedes aegypti from the State of São Paulo, Brazil, to Organophosphates Insecticides. Memórias do Instituto Oswaldo Cruz, 98, 703-708.
Monnerat, R. G.; Soares, C. M. S. ; Gomes, A. C. M.; Jones, G.; Martins, E.; Praça, L.; Berry, C. (2009), Translocation and insecticidal activity of Bacillus thuringiensis bacteria living inside of plants. Microbial Biotechnology, 2, 1560-1562.
Monnerat, R. G.; Silva, S. F.; Silva-Werneck, J. (2001), O catálogo do banco de germoplasma de bactéria entomopatogênica do gênero Bacillus. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 65p.
Petry, F.; Lozovei, A. L.; Ferraz, M. E.; Santos Neto, L. G. (2004), Controle integrado de espécies de Simulium (Diptera, Simuliidae) por Bacillus thuringiensis e manejos mecânicos no riacho e nos vertedouros de tanques de piscicultura, Almirante Tamandaré, Paraná, Brasil. Revista Brasileira de Entomologia, 48, 127-132.
Poupardin, R.; Reynaud, S.; Strode, C.; Ranson, H.; Vontas, J.; David, J. P. (2008), Crossinduction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti: impact on larval tolerance to chemical insecticides. Insect Biochemistry and Molecular Biology, 38, 540-551.
Prabakaran, G.; Hoti, S. L.; Manonmani, A. M.; Balaraman, K. (2008), Coconut water as a cheap source for the production of endotoxin of Bacillus thuringiensis var. israelensis, a mosquito control agent. Acta Tropica, 105, 35-38.
Sosa-Gómez, D. R.; Tigano, M. S.; Alves, S. B. (1998), Caraterização de entomopatógenos In: Alves, S.B. Controle microbiano de insetos. São Paulo: FEALQ, 22, 731-764.
Schnepf, E.; Crickmore, N.; Van Rie, J.; Lereclus, D.; Baum, J.; Feitelson, J.; Zeigler, D. R.; Dean, D. H. (1998), Bacillus thuringiensis and its pesticide crystal proteins. Microbiology and Molecular Biology Reviews, 62, 775-806.
Tikar, S. N.; Kumar, A.; Prasad, G. B.; Prakash, S. (2009), Temephos-induced resistance in Aedes aegypti and its cross-resistance studies to certain insecticides from India. Parasitology. Reserch, 105, 57-63.
Uribe, D.; Marinez, W.; Cerón, J. (2003), Distribution and diversity of cry genes in native strains of Bacillus thuringiensis obtained from different ecosystems from Colombia. Journal of Invertebrate Pathology, 82, 119-127.
Vilarinhos, P. T. R.; Dias, J. M. C. S.; Andrade, C. F. S.; Araújo- Coutinho, C. J. P. C. (1998), Uso de bactérias para o controle de culicídeos e simulídeos. In: Alves, S.B. Controle microbiano de insetos. Piracicaba: Fundação de Estudos Agrários Luiz de Queiroz, 80-447p.
Vilarinhos, P. T. R. and Monnerat, R. G. (2004), Larvicidal persistance of Bacillus thuringiensis israelensis formulations to control Aedes aegypti larvae. Journal of Ayub Medical College, 20, 311- 314.
Yousten, A. A. (1984), Bacillus sphaericus: microbiological factors related to its potential as a mosquito larvicide. Advances in Biotechnology Processes, 3, 315-343.
WHO [World Health Organization]. (1999), Microbial pest control agent Bacillus thuringiensis. Enviromental Health Criteria. Geneva, Switzerland: World Health Organization, 217p.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 - Journal of Biotechnology and Biodiversity
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0 at http://creativecommons.org/licenses/by/4.0/) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Available at The Effect of Open Access, at http://opcit.eprints.org/oacitation-biblio.html).
