DNA extraction and molecular identification of Olivea neotectonae (T.S. Ramakrishnan & K. Ramakrish-nan) isolated from teak leaves (Tectona grandis L.f).
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v9n2.leaoKeywords:
forest pathology, rust-causing fungus, Pucciniales, sequencing, ITS regionAbstract
Phytosanitary problems can pose a threat in different crops. And, for this, the first step is to identify and know the main diseases and their impacts on forest stands, such as teak (Tectona grandis L.f). Currently, the rust caused by Olivea neotectonae (T.S. Ramakrishnan & K. Ramakrishnan) is one of the main fungal diseases that affect the species. The objective of this work was to characterize O. neotectonae isolated from teak leaves in the state of Tocantins. The urediniospores of O. neotectonae were collected from naturally infected teak leaves in the city of Gurupi-TO. Three different methods were tested, to verify the one that best provided obtaining DNA with sufficient quantity and adequate quality for the correct identification of the pathogen. The method that resulted in the best quantity and quality of DNA from isolates was that described by Alessio and collaborators. The extracted DNA was subjected to amplification of the ITS region and sequenced. The nucleotide sequence of O. neotectonae shared a similarity of about 70% with sequences from the ITS region of other fungi deposited in the GenBank, since until now there are no available sequences of the species O. neotectonae.
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