Análisis de la expresión del gen FRIGIDA4-like (FRL4) de café (Coffea arábica L.)

Autores/as

  • Matheus Martins Daude 1Laboratory of Molecular Analyzes, Graduate Program Master in Health Sciences, Federal University of Tocantins, Palmas, Brazil
  • André Almeida Lima 2Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil.
  • Antonio Chalfun Junior 2Laboratory of Molecular Plant Physiology, Department of Biology, Federal University of Lavras, Lavras, Brazil.
  • Horllys Gomes Barreto Universidade Federal do Tocantins

DOI:

https://doi.org/10.20873/uft.2359-3652.2018v5nEspecialp204

Resumen

ABSTRACT

Coffee is one of the most economically important commodities. In Brazil, this crop is responsible for generating more than eight million jobs. In the foreign market, Brazil is the largest producer and exporter of coffee. Due to its economic importance, several studies aiming the improvement of coffee are conducted, but there are still problems related to its productivity and quality of the beverage, such as sequential flowering, which causes production losses and a low quality drink. Thus, understanding the molecular mechanisms involved in the flowering process is essential to elucidate how flowering occurs in the coffee crop. The FRI gene is one of the main genes involved in flowering, as it positively regulates the FLC gene at expression levels that inhibit flowering. Thus, the objective of this work was to identify and analyze the expression of the FRIGIDA4-like gene (FRL4) through Bioinformatics and real-time PCR (RT-qPCR). The CaFRL4 gene was identified and showed high expression levels in leaf during flowering, which corroborates with the literature. The results obtained provide the basis for future studies involving genetic transformation in model plants and coffee, permitting the functional characterization of this gene.

Keywords: RT-qPCR, Arabica coffee, Flowering.

Citas

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Publicado

2018-10-31

Cómo citar

Daude, M. M., Lima, A. A., Chalfun Junior, A., & Barreto, H. G. (2018). Análisis de la expresión del gen FRIGIDA4-like (FRL4) de café (Coffea arábica L.). DESAFIOS, 5(Especial), 204–213. https://doi.org/10.20873/uft.2359-3652.2018v5nEspecialp204