CHANGES IN BLOOD PARAMETERS AND LIVER MORPHOPHYSIOLOGY IN NILE TILAPIA, OREOCHROMIS NILOTICUS, AFTER ACUTE EXPOSURE WITH BACILLUS THURINGIENSIS-BASED BIOINSECTICIDE

Authors

DOI:

https://doi.org/10.20873/DEZ_23_03

Abstract

Biopesticides aim to replace synthetic pesticides, and those formulated based on Bacillus thuringiensis (Bt) are the most used in crops. However, little is known about its mechanism of action and effects on the aquatic biota. The objective was to evaluate the sublethal effects of Bt in Nile tilapia after acute exposure. The fish were divided into a control group (GC), one exposed to 30 mg L-1 of the biopesticide (G30) and one exposed to 40 mg L-1 of Bt (G40), for 48 hours. After exposure, blood and liver samples were collected for toxicity analysis. The number of erythrocytes with lobulated and kidney-shaped nuclei increased, but without observing micronuclei. There was no alteration in the erythrocyte parameters, however, the number of total leukocytes and thrombocytes increased in the treated ones. Hepatic histopathological changes were evidenced by an increase in intracellular inclusions, nuclear alterations and a decrease in hypertrophied hepatocytes among those treated. The Iorg demonstrated impairment of the hepatic tissue in the G30 group in relation to the control and among the treated. Damage resulting from exposure to Bt can be severe, even though it is categorized as reversible. Low concentrations of Bt can induce deleterious effects for fish, interfere with the immune and cytological systems of the blood and liver.

Published

2023-12-28

How to Cite

do Carmo da Silva, D., Rubens Rodrigues Ferreira, E., Estevan Moron, S., dos Santos Mariano, W., & Paulino, M. G. (2023). CHANGES IN BLOOD PARAMETERS AND LIVER MORPHOPHYSIOLOGY IN NILE TILAPIA, OREOCHROMIS NILOTICUS, AFTER ACUTE EXPOSURE WITH BACILLUS THURINGIENSIS-BASED BIOINSECTICIDE. DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 10(3). https://doi.org/10.20873/DEZ_23_03