RESÍDUO DE FLUOROQUINOLONA EM CARNE DE FRANGO E EFEITO DA MISTURA DE FEZES NA RESISTÊNCIA DE Salmonella Heidelberg À ENROFLOXACINA

Resíduo de fluoroquinolona em carne de frango

Autores

  • Fernanda Zonatto UTFPR
  • Debora Francielly Oliveira UNIR
  • Eder Costa dos Santos
  • Elisabete Hiromi Hashimoto Universidade Tecnológica Federal do Paraná

DOI:

https://doi.org/10.20873/uftv9-11505

Resumo

The objective of this work was to validate a methodology for the detection of enrofloxacin and in chicken tissues and to investigate the influence of the mixture of chicken feces on the resistance of Salmonella spp. to enrofloxacin. Enrofloxacin (10 mg.kg-1) was administered orally, tissues (muscle, liver and kidney) were collected until the 10th day of the last dose and analyzed by LCMS. A total of 124 feces samples were collected from poultry litter and Salmonella spp were isolated and in parallel, feces were added of a solution containing enrofloxacin (10 μg.mL-1) followed by Salmonella spp. isolation and identification. The limit of quantification and detection of the LCMS was 20 and 10 μg.kg-1, respectively, and the withdrawal period was 3 days. When exposed to enrofloxacin in the presence of a fecal mixture, Salmonella Heidelberg showed less susceptibility (39.8%) to enrofloxacin than when exposed alone (100%). Considering all the validation parameters analyzed, this LCMS was sensitive and applicable to the routine analysis of fluoroquinolone residues. Differences in susceptibility to enrofloxacin in the presence of fecal material indicate the need to consider environmental conditions in the resistance of pathogens, mainly to the classes of antimicrobials used in veterinary and human medicine.

Biografia do Autor

Fernanda Zonatto, UTFPR

Mestre em Tecnologia de Alimentos.

Debora Francielly Oliveira, UNIR

Professora do Departamento de Engenharia de Alimentos, Universidade Federal de Rondônia - UNIR

Eder Costa dos Santos

Professor do Depto Acadêmico de Engenharia Agronômica do Câmpus Francisco Beltrão, da UTFPR

Downloads

Publicado

2022-05-24

Como Citar

Zonatto, F. ., Oliveira, D. F. ., Costa dos Santos, E., & Hashimoto, E. H. (2022). RESÍDUO DE FLUOROQUINOLONA EM CARNE DE FRANGO E EFEITO DA MISTURA DE FEZES NA RESISTÊNCIA DE Salmonella Heidelberg À ENROFLOXACINA: Resíduo de fluoroquinolona em carne de frango. DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 9(2), 179–190. https://doi.org/10.20873/uftv9-11505

Edição

Seção

Artigos