Protocol suitability for in vitro culture of ‘Xingu’ blackberry (Rubus sp.)

Authors

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v8n2.schiehl

Keywords:

tissue culture, cytokinin, auxin

Abstract

In vitro production of blackberry seedlings makes it possible to obtain seedlings quickly and healthy. The objective of this research was to optimize a micropropagation protocol of the 'Xingu' blackberry, defining the most efficient BAP concentration for multiplication, the best gelling agent and to verify the need for IBA in ex vitro rooting and survival during acclimatization. BAP concentrations (0, 2.5, 5, 7.5 and 10 µM) were evaluated by 3 subcultures. The gelling agents evaluated were Gelrite® (2 g L-1), Vetec® brand agar (7 g L-1) and liquid medium. Rooting of shoots, treated with IBA concentrations (0; 2.5; 4.9 and 9,8mM), was performed ex vitro in a nebulization chamber. There was an increase in the amount of shoots formed with increasing BAP concentration, up to 5.0 µM. Agar was the gelling agent that generated higher quality explants and less hyperhydricity. Rooting and survival rates of ex vitro explants were 100% in all evaluated treatments. The blackberry cv. Xingu can be micropropagated in MS culture medium plus 5 µM BAP and solidified with agar (7 g L-1) and rooting can be performed ex vitro during acclimatization without the need for auxins.

References

Antunes LEC, Pereira IS, Picolotto L, Vignolo GK, Gonçalves MA. Produção de amoreira-preta no Brasil. Revista Brasilei-ra de Fruticultura, v.36, n.1, p.100-111, 2014.

http://dx.doi.org/10.1590/0100-2945-450/13

Aremu AO, Bairu MW, Dolezal K, Finnie JF, Staden JV. Topolins: A panacea to plant tissue culture challenges? Plant Cell Tissue and Organ Culture, v.108, p.1-16, 2012.

http://dx.doi.org/10.1007/s11240-011-0007-7

Arencibia AD, Vergara C, Quiroz K, Carrasco B, García-Gonzalez R. Establishment of photomixotrophic cultures for raspberry micropropagation in Temporary Immersion Biore-actors (TIBs). Scientia Horticulturae, Amsterdan, v.160, p.49-53, 2013.

https://doi.org/10.1016/j.scienta.2013.05.010

Augusto CSS, Biasi LA, Telles CA. Enraizamento e aclimati-zação de plantas micropropagadas de amoreira-preta cv. Brazos. Revista Brasileira de Fruticultura, Jaboticabal, v.28, n.3, p.473-476, 2006.

http://dx.doi.org/10.1590/S0100-29452006000300029

Beck SL, Dunlop R, Staden Van J. Rejuvenation and micro-propagation of adult Acacia mearnsii using coppice material. Plant Growth Regulation, Dordrecht, v.26, p.149-153, 1998.

Bueno PMC, Biasi LA. Micropropagation of greenberry (Rubus erythroclados). Acta Horticulturae, n.1083, p.383-389, 2015.

http://dx.doi.org/10.17660/ActaHortic.2015.1083.48

Campbell MM, Brunner AM, Jones HM, Strauss, SH.; For-estry’s fertile crescent: the application of biotechnology to forest trees. Plant Biotechnology Journal, v.1, n.3, p.141–154, 2003.

http://dx.doi.org/10.1046/j.1467-7652.2003.00020.x

Croge CP, Cuquel FL, Biasi LA, Bona CM. Performance of blackberry cultivars in Cerro Azul-PR. Revista Brasileira de Fruticultura, v.38, n.3, 2016.

http://dx.doi.org/10.1590/0100-29452016141

Croge CP, Cuquel FL, Biasi LA, Bona CM, Pintro PTM. Agronomic performance of blackberry cultivars in Lapa-PR. Revista Brasileira de Fruticultura, v.41, n.2, 2019.

http://dx.doi.org /10.1590/0100-29452019101

Croge CP, Cuquel FL, Pintro PM, Biasi LA, Bona CM. Anti-oxidant Capacity and polyphenolic compounds of blackber-ries produced in different climates, HortScience, v.54, n.12, p.2209-2213, 2019.

https://doi.org/10.21273/HORTSCI14377-19

Dias JPT, Takahashi K, Duarte Filho J, Ono EO. Bioestimu-lante na promoção da brotação em estacas de raiz de amorei-ra-preta. Revista Brasileira de Fruticultura, Jaboticabal, v.34, n.1, p.1-7, 2012.

http://dx.doi.org/10.1590/S0100-29452012000100003

Erig AC, Rossi A, Fortes GRL. 6-benzilaminopurina e ácido indolbutírico na multiplicação in vitro da amoreira-preta (Rubus idaeus L.), cv. Tupy. Ciência Rural, Santa Maria, v.32, n.5, p.42-46, 2002.

http://dx.doi.org/10.1590/S0103-84782002000500005

Kefayeti S, Kafkas E, Ercisl S. Micropropagation of ‘Chester thornless’ blackberry cultivar using axillary bud explants. Notulae Botanicae Horti Agrobotanici Cluj-napoca. v.47, n.1, p.162-168, 2019.

http://dx.doi.org/10.15835/nbha47111280

Leitzke L, Damiani CR, Schuch MW. Influência do meio de cultura, tipo e concentração de citocininas na multiplicação in vitro de amoreira-preta e framboeseira. Ciência e Agrotecno-logia, Lavras, v.34, n.2, p.352-360, 2010.

http://dx.doi.org/10.1590/S1413-70542010000200012

Machado MP, Biasi LA, Ritter M, Ribas LLF, Koehler HS. Multiplicação in vitro do porta-enxerto de videira VR043-43 (Vitis vinifera x Vitis rotundifolia). Ciência e Agrotecnolo-gia, Lavras, v.30, n.4, p.648-655, 2006.

http://dx.doi.org/10.1590/S1413-70542006000400009

Mengarda LHG, Povoas L, Debiasi C, Pescador R. Estado físico na propagação in vitro de Bromeliaceae. Scientia Agraria, Curitiba, v.10, n.6, p.469-474, 2009.

http://dx.doi.org/10.5380/rsa.v10i6.15532

Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, Copenhagen, v.15, p.473-497,1962.

http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nascimento AC, Paiva R, Nogueira RC, Porto JMP, Nogueira GF, Soares FP. BAP e AIB no cultivo in vitro de Eugenia pyriformis Cambess. Revista Acadêmica: Ciências Agrárias e Ambientais, Curitiba, v.6, n.2, p.223-228, 2008.

Oliveira MKT, Bezerra Neto F, Câmara FAA, Nunes GHS, Oliveira FA. Propagação in vitro da cultura do abacaxizeiro Ornamental (Ananas lucidus Miller). Revista Caatinga, v.20, n.3, p.167-171, 2007.

Oliveira RP, Nino AFP, Ferreira VF. Potencial de multiplica-ção in vitro de cultivares de amoreira-preta. Revista Brasilei-ra de Fruticultura, Jaboticabal, v.30, n.3, p.585-589, 2008.

http://dx.doi.org/10.1590/S0100-29452008000300004

Pasa MS, Carvalho GL, Schuch MW, Schmitz JD, Torchelsen MM, Nickel GK, Sommer LR, Lima TS, Camargo SS. Qua-lidade de luz e fitorreguladores na multiplicação e enraiza-mento in vitro da amoreira-preta ‘Xavante’. Ciência Rural, Santa Maria, v.42, n.8, p.1392-1396, 2012.

https://doi.org/10.1590/S0103-84782012000800010

Pelizza TR, Silveira FN, Ribeiro RS, Machado BD, Rufatto L. In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'. Ciência Rural, Santa Maria, v.46, n.9, p.1542-1545, 2016.

https://doi.org/10.1590/0103-8478cr20140988

Pereira GA, Boliani AC, Corrêa LS. Concentrações de 6-Benzilaminopurina (BAP) e Cinetina (CIN) e tempos de cul-tivo na micropropagação de bananeira ‘Thap maeo’. Plant Cell Culture e Micropropagation, Lavras, v.10, n.1, p.7-12, 2014.

Pereira JES, Fortes GRL. Protocolo para produção de material propagativo de batata em meio líquido. Pesquisa Agropecuá-ria Brasileira, Brasília, v.38, n.9, p.1035-1043, 2003.

http://dx.doi.org/10.1590/S0100-204X2003000900003

Radmann EB, Bianchi VJ, Souza TM, Fachinello JC, Oliveira RP. Influência da composição do meio de cultivo e do tipo de explante na micropropagação do porta-enxerto de Prunus sp. ‘GXN-9’. Scientia Agraria, Curitiba, v.10, n.2, p.095-101, 2009.

http://dx.doi.org/10.5380/rsa.v10i2.13573

Raseira MCB, Franzon RC, Scaranari C. Cultivar de amora-preta BRS Xingu: alternativa a cultivar Brazos para o Sudes-te do Brasil. Pelotas: Embrapa Clima Temperado. 2018. 6p. (Comunicado Técnico, 362).

Rescarolli CL, Zafarri GR. Produção de mudas de Etlingera elatior através da cultura de tecidos vegetais in vitro. Orna-mental Horticulture, Itajaí, v. 13 s/p. 2007.

https://doi.org/10.14295/oh.v13i0.1601

Rodrigues FA, Penoni ES, Soares JDR, Pasqual M. Diferen-tes concentrações de sais do meio MS e BAP na multiplica-ção in vitro de Physalis peruviana L. Bioscience Journal, Uberlândia, v.29, n.1, p.77-82, 2013.

Santos BR, Paiva R, Nogueira RC, Oliveira LM, Silva DPC, Martinotto C, Soares FP, Paiva PDO. Micropropagação de pequizeiro (Caryocar brasiliense Camb.). Revista Brasileira de Fruticultura, Jaboticabal, v.28, n.2, p.293-296, 2006.

http://dx.doi.org/10.1590/S0100-29452006000200031

Schuchovski CS, Biasi LA. Development of an efficient pro-tocol for ‘Brazos’ blackberry in vitro multiplication. Acta Horticulturae, v.1224, p.157-164, 2018.

https://doi.org/10.17660/ActaHortic.2018.1224.21

Silva AB, Pasqual M, Maciel ALR, Dutra LF. BAP e substra-tos na aclimatização de plântulas de gloxínia (Sinningia spe-ciosa Lood. Hiern.) provenientes de cultura de tecidos. Ci-ência e Agrotecnologia, Lavras, v.27, n.2, p.255-260, 2003.

http://dx.doi.org/10.1590/S1413-70542003000200002

Toledo JA, Biasi LA. Multiplicação e enraizamento in vitro da amoreira preta cv. Xavante. Cultura Agronômica, Ilha Sol-teira, v.27, n.3, p.328-339, 2018.

Vasconcelos AGV, Tomas LF, Camara TR, Willadino L. Hiperidricidade: uma desordem metabólica. Ciência Rural, Santa Maria, v.42, n.5, p.837-844, 2012.

http://dx.doi.org/10.1590/S0103-84782012000500013

Villa F, Araújo AG, Pio LAS, Pasqual M. Multiplicação in vitro da Amoreira-preta 'Ébano' em diferentes concentrações de meio MS e BAP. Ciência e Agrotecnologia, v. 29, n. 3, p. 582-589, 2005.

http://dx.doi.org/10.1590/S1413-70542005000300011.

Villa F, Fráguas CB, Dutra LF, Pio LAS, PASQUAL M. Multiplicação in vitro de amoreira-preta cultiva Brazos. Ci-ência e Agrotecnologia, Lavras, v.30, n.2, p.266-270, 2006.

http://dx.doi.org/10.1590/S1413-70542006000200011

Villa F, Pasqual M, Assis FA, PIO LAS, Assis GA. Cresci-mento in vitro de amoreira-preta: efeito de reguladores de crescimento e da cultivar. Ciência e Agrotecnologia, Lavras, v.32, n.6, p.1754-1759, 2008.

http://dx.doi.org/10.1590/S1413-70542008000600012

Vizzotto M, Raseira MCB, Pereira MC, Fetter MR. Teor de compostos fenólicos e atividade antioxidante em diferentes genótipos de amoreira-preta (Rubus sp.). Revista Brasileira de Fruticultura, Jaboticabal, v.34, n.3, p.853-858, 2012.

http://dx.doi.org/10.1590/S0100-29452012000300027

Wang SY, Maas JL, Payne JA, Galleta GJ. Ellagic acid con-tent in small fruits mayhaws, and other plants. Journal Small Fruit and Viticulture, Louisiana, v.2, n.4, p.11-49, 1994.

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Published

2020-06-25

How to Cite

Schiehl, M., França, T. O. de, & Biasi, L. A. (2020). Protocol suitability for in vitro culture of ‘Xingu’ blackberry (Rubus sp.). Journal of Biotechnology and Biodiversity, 8(2), 079–087. https://doi.org/10.20873/jbb.uft.cemaf.v8n2.schiehl

Issue

Vol. 8 No. 2 (2020): Journal of Biotechnology and Biodiversity

Section

Biotechnology

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