Influence of growth regulators and explant type on in vitro morphogenesis of Hancornia speciosa Gomes

Authors

  • Kívia Soares de OLIVEIRA Universidade Federal do Rio Grande do Norte
  • Fúlvio Aurélio de Morais Freire Rio Grande do Norte Federal University
  • Magdi Ahmed Ibrahim Aloufa Rio Grande do Norte Federal University

DOI:

https://doi.org/10.20873/uftv6-6459

Abstract

Mangabeira (Hancornia speciosa Gomes), belonging to the family Apocynaceae family, is a native species of the Cerrado and the Northeast coastal plains, and has great socio-economic, environmental and cultural importance. The objective of this study was to evaluate the influence of different concentrations of 6-benzylaminopurine (BAP) and indole butyric acid (AIB) on the in vitro morphogenesis of H. speciosa. For this, apical and nodal explants were inoculated into basic MS medium and into MS medium supplemented with 1.5; 2.5 and 3.5mg.L-1 BAP to 1.25; 2.5 and 3.0 mg.L-1 of AIB. The variables observed were: sprout formation, number of shoots per explant, number of nodes per shoot, contamination, survival, root formation, mean number of roots and callus presence. It was found that explants of shoot apices in the absence of BAP were more responsive in relation to the rate of in vitro multiplication and induction of shoots. Rhizogenesis from shoot apices and nodal segments was not favored in the IBA concentrations used. However, the concentration of 3.0mg.L-1 AIB induces the formation of more roots, increases calogênse and survival of nodal and apical segments of H. speciosa.

Keywords: Apocynaceae; cytokinin; auxin.

Author Biographies

Fúlvio Aurélio de Morais Freire, Rio Grande do Norte Federal University

Professor Adjunto do Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte, Brasil.

Magdi Ahmed Ibrahim Aloufa, Rio Grande do Norte Federal University

Professor Adjunto do Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte, Brasil.

Published

2019-12-04

How to Cite

OLIVEIRA, K. S. de ., Freire, F. A. de M., & Aloufa, M. A. I. (2019). Influence of growth regulators and explant type on in vitro morphogenesis of Hancornia speciosa Gomes. DESAFIOS - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 6(4), 60–74. https://doi.org/10.20873/uftv6-6459

Issue

Section

Artigos