Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v2n3.alcantaraPalabras clave:
Disinfestation of seeds, water-alcohol solution, sodium hypochlorite, in vitro cultureResumen
Many technical and scientific studies have been carried out for eucalyptus cultivation, especially in the area of large-scale clonal propagation. However, in some studies it is necessary to obtain aseptic materials via seeds, and for this purpose there are no efficient aseptic protocols. Given this problem, the present study aimed to define a protocol for asepsis of Eucalyptus grandis seeds for establishment and germination in vitro. Four aseptic methods were tested using benomyl-based fungicide, hydroalcoholic solution (70% v / v) and sodium hypochlorite (NaOCl), alternating the order of application. The experiment was conducted in a completely randomized design with five replications. The percentage of establishment and contamination were evaluated at the end of the experiment. The best method found was the sterile water wash sequence followed by 70% alcohol for 30 s, 2.5% sodium hypochlorite for 20 min, 1% benomyl fungicide for 20 min and three washes with sterile water, corresponding to 87.5% of establishment.
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Aggarwal, D.; Kumar, A.; Reddy, M. S. (2010), Shoot organogenesis in elite clones of Eucalyptus tereticornis. Plant Cell Tissue and Organ Culture, Netherlands, 102, 45-52.
Alves, E. C. S. C.; Xavier, A.; Otoni, W. C. (2004), Organogênese in vitro a partir de explante caulinar na regeneração de clones de Eucalyptus grandis W. Hill ex Maiden x E. urophylla S. T. Blake. Revista Árvore, 28, 643-653.
Azevedo, R. A. and Arruda, P. (2010), High-lysine maize: the key discoveries that have made it possible. Amino Acids, 39, 979-989.
Azevedo, R. A.; Arruda, P.; Turner, W. L.; Lea, P. J. (1997), The biosynthesis and metabolism of the aspartate derived amino acids in higher plants. Phytochemistry, 46, 395-419.
Bandeira, F. S.; Xavier, A.; Otoni, W. C.; Dias, J. M. M. (2006), Enxertia in vitro na propagação de clones de Eucalyptus urophylla e E. grandis. Pesquisa Agropecuária Brasileira, 41, 223-232.
Basso, L. H. M.; Lima, G. P. P.; Gonçalves, A. N.; Vilhena, S.M.C.; Padilha, C.C.F. (2007), Effect of aluminum on the free polyamines content and acid phosphatase activity during the growth of Eucalyptus grandis x E. urophylla shoots cultivated in vitro. Scientia Forestalis, 75, 9-18.
Bonato, C. M.; Rubin Filho, C. J.; Melges, E.; Santos, V. D. (1998), Nutrição Mineral de Plantas. Maringá: Universidade Estadual de Maringá. 60p.
Boughalleb, N.; Tarchoun, N.; Dallagi, W. (2006), Effect of fungicides on in vitro infestation level of radish, carrot and pepper seeds. Plant Pathology Journal, 5, 388-392.
Brondani G. E.; Dutra, L. F.; Grossi, F. G.; Wendling, I.; Azevedo, J. H. (2009), Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden. Revista Árvore, 33, 11-19.
Brondani, G. E.; Hansel, F. A.; Dutra, L. F.; Wendling, I. (2010), Desinfestação e meio de cultura para o estabelecimento in vitro de segmentos nodais de Liquidambar styraciflua. Floresta, 40, 541-554.
Carnegie, A. J. and Ades, P. K. (2003), Mycosphaerella leaf disease reduces growth of plantation-grown Eucalyptus globulus. Australian Forestry, 66, 113-119.
Carrero, C.; Cedeño, L.; Quintero, K.; Pino, H.; Rodríguez, L. (2003), Identification and in vitro sensitivity to fungicides of the causal agent of stem rot on Eucalyptus cinerea seedlings in Mérida, Venezuela. Interciencia, 28, 656-659.
Dutra, L. F.; Wendling, I.; Brondani, G. E. (2009), A micropropagação do eucalipto. Pesquisa Florestal Brasileira, 58, 49-59.
Embrapa. Empresa Brasileira de Pesquisa Agropecuária. (2009), Programa SOC - Software Científico, Versão 2.1, Embrapa Informática Agropecuária, Campinas.
Hossain, H. M. M. T.; Kim, Y. H.; Lee, Y. S. (2010), The apical bud as a novel explant for high- frequency in vitro plantlet regeneration of Perilla frutescens L. Britton. Plant Biotechnology Reports, 4, 229-235.
Huang, Z. C.; Zeng, F. H.; Lu, X. Y. (2010), Efficient regeneration of Eucalyptus urophylla from seedling-derived hypocotyls. Biologia Plantarum, 54, 131-134.
Hung, C. D. and Trueman, S. J. (2010), Nutrient responses differ between node and organogenic cultures of Corymbia torelliana x C. citriodora (Myrtaceae). Australian Journal of Botany, 58, 410-419.
Lisboa-Padulla, T.; Moraes, M. H. D.; Menten, J. O. M.; Barbedo, C. (2009), Tratamento de sementes de pau-brasil com fungicidas: efeito na incidência de fungos, germinação e transmissão de fungos pelas sementes. Summa Phytopathologica, 35, 148-150.
Murashige, T. and Skoog, F. A. (1962), Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 3, 473-497.
Netto, D. A. M. and Faiad, M. G. R. (1995), Viabilidade e sanidade de sementes de espécies florestais. Revista Brasileira de Sementes, 17, 75- 80, 1995.
Ray, R. C. (1984), Effect of some pesticide combinations on sulfur oxidation in the simulated oxidized layer of a flooded soil. Environmental Pollution, 34, 15-22.
Silva, A. L. L.; Oliveira, Y.; Costa, J. L.; Masetto, E.; Mudry, C. S.; Erasmo, E. A. L.; Scheidt, G. N. (2010), Shoot tip and cotyledon explants of Eucalyptus saligna Sm. cultivated on different kanamycin levels. Journal of Biotechnology and Biodiversity, 1, 1-5.
Silva, A. L. L.; Oliveira, Y.; Costa, J. L.; Mudry, C. S.; Procopiuk, M.; Scheidt, G.N.; Brondani, G. E. (2011), Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, 2, 1-6.
Souza, G. M.; Gonçalves, A. N.; Machado Neto, N. B. (1999), Crescimento in vitro de progênies de Eucalyptus camaldulensis Dehn. sob condições de deficiência hídrica. Scientia Agricola, 56, 557-562.
Yang, H. J. (1976), Effect of benomyl on Asparagus officinalis L. shoot and root development in culture media. Horticultural Science, 11, 473-474.
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