Multiplicação, alongamento e enraizamento de brotações in vitro de clones de cana-de-açúcar submetidos a diferentes concentrações de 6-benzilaminopurina e ácido giberélico

Giovana Bomfim de Alcantara; Marília Pereira Machado; Daniel de Sousa Ribeiro; Haline Helena Wippel; João Carlos Bespalhok Filho; Ricardo Augusto de Oliveira; Edelclaiton Daros; Giovana Bomfim de Alcantara; Marília Pereira Machado; Daniel de Sousa Ribeiro; Haline Helena Wippel; João Carlos Bespalhok Filho; Ricardo Augusto de Oliveira; Edelclaiton Daros

doi:10.20873/jbb.uft.cemaf.v5n1.alcantara

Shoot multiplication, elongation and rooting in vitro of clones of sugarcane under different concentrations of 6-benzylaminopurine and gibberellic acid

Authors

Giovana Bomfim de Alcantara
Marília Pereira Machado
Daniel de Sousa Ribeiro
Haline Helena Wippel
João Carlos Bespalhok Filho
Ricardo Augusto de Oliveira
Edelclaiton Daros
Giovana Bomfim de Alcantara Universidade Federal do Paraná
Marília Pereira Machado Universidade Federal do Paraná https://orcid.org/0000-0002-9634-3192
Daniel de Sousa Ribeiro Universidade Federal do Paraná
Haline Helena Wippel Universidade Federal do Paraná
João Carlos Bespalhok Filho Universidade Federal do Paraná https://orcid.org/0000-0003-2705-9750
Ricardo Augusto de Oliveira Universidade Federal do Paraná https://orcid.org/0000-0002-7472-9238
Edelclaiton Daros Universidade Federal do Paraná https://orcid.org/0000-0003-0258-740X

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v5n1.alcantara

Keywords:

Saccharum L., micropropagation, meristems, seedling production

Abstract

The culture of meristems allows obtaining plant sugarcane healthy with genetic stability and enables the production of a large number of seedlings during the year. The aim of this work was to establish suitable conditions for seedling production in vitro by meristems of three potential clones sugarcane (RB036091, RB036152 and RB036066). The multiplication and elongation of shoot buds were evaluated. The BAP (0, 0.444, 0.888 and 1.776 mM) was added in the medium for multiplication of shoot buds and GA3 (0, 0.3 and 0.6 mM) for elongation of shoot buds. Presence of BAP in the medium had an inhibitory influence on shoot buds height. The BAP in the medium promoted the tillering for the three clones. The GA3 increased the height of shoots for clones RB036152 and RB036066. The GA3 promoted the formation of a greater plant, however, fewer roots were formed for all clones tested. It was concluded that the concentration of 0.444 mM BAP promotes the multiplication of clones RB036091, RB036066 and RB036052. The clone RB036091 showed the higher multiplication rate. The GA3 promotes the elongation of the seedlings for clones RB036152 and RB036066.

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Published

2014-02-04

How to Cite

de Alcantara, G. B., Machado, M. P., Ribeiro, D. de S., Wippel, H. H., Bespalhok Filho, J. C., de Oliveira, R. A., … Daros, E. (2014). Shoot multiplication, elongation and rooting in vitro of clones of sugarcane under different concentrations of 6-benzylaminopurine and gibberellic acid. Journal of Biotechnology and Biodiversity, 5(1), 20–25. https://doi.org/10.20873/jbb.uft.cemaf.v5n1.alcantara

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Issue

Vol. 5 No. 1 (2014): Journal of Biotechnology and Biodiversity

Section

Biotechnology

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