Genetic selection of Calophyllum brasiliense for seed orchards
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v4n4.schuhliKeywords:
RAPD, propagative material, MAS, guanandiAbstract
Calophyllum brasiliense populations are under severe depletion and criteria to improve production and quality of propagative material are therefore necessary. Genetic measures have the potential to reduce consanguinity and maximize allelic representation within target populations. Here, we explored genetic values for this species in a small relic of natural forest in Rio de Janeiro State (Brazil). The objective was to evaluate the potential of some genetic measures for seed orchard establishment. As genomic information of native trees is still scarce, we opted to use a dominant marker: RAPD. DNA from 17 phenotypically superior trees was obtained through the CTAB method and submitted for amplification by PCR. Electrophoresis and electronic documentation was then conducted. We calculated the percentage of polymorphic bands (PPB), gene diversity (Ht), Shannon’s information index (i), genetic distance (UPGMA) and parsimony analysis. Six primers were evaluated generating 34 loci. We found high genetic diversity PPB=70.6% with Ht=0.28 and i=0.41. Genetic relationships were reported in dendrograms (maximum parsimony and distance). Simulated sampling within and among clusters suggests that inter cluster sampling is more effective to capture the genetic diversity.
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