Plant regeneration of sugarcane cv. RB931003 and RB98710 from somatic embryos and acclimatization

Authors

  • Roberson Dibax Universidade Federal do Sergipe
  • Giovana Bomfim de Alcântara Universidade Federal do Paraná
  • João Carlos Bespalhok Filho Universidade Federal do Paraná https://orcid.org/0000-0003-2705-9750
  • Marilia Pereira Machado Universidade Federal do Paraná
  • Yohana de Oliveira Universidade Federal do Paraná
  • André Luís Lopes da Silva Universidade Federal do Paraná

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v2n3.dibax

Keywords:

Saccharum spp., 6-benzylaminopurine, ussue culture, somatic embryogenesis

Abstract

The aim of this study was to establish a protocol for regeneration and acclimatization of sugarcane (RB931003 and RB98710) cultivars from somatic embryos. For the cultivar RB931003, the concentration of 0.25 mg L-1 BAP was efficient and provided the shoot induction in 80% of embryogenic masses. For the cultivar RB98710, the highest percentage of shoot induction was observed in 0 and 0.25 mg L-1 BAP, which promoted 70% and 75% of shoots from embryogenic masses, respectively. The MS medium without growth regulators proved to be appropriate for elongation and rooting of shoots and the use of substrate composed by vermiculite and MS salts was effective in the acclimatization of plants for both cultivars analyzed.

References

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Published

2011-08-08

How to Cite

Roberson, Giovana Bomfim, João Carlos, Marília Pereira, Yohana, André Luís Lopes, … Silva, A. L. L. da. (2011). Plant regeneration of sugarcane cv. RB931003 and RB98710 from somatic embryos and acclimatization. Journal of Biotechnology and Biodiversity, 2(3), 32–37. https://doi.org/10.20873/jbb.uft.cemaf.v2n3.dibax

Issue

Vol. 2 No. 3 (2011): Journal of Biotechnology and Biodiversity

Section

Biotechnology

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