Shoot tip and cotyledon explants of Eucalyptus saligna Sm. cultivated on different kanamycin levels

Authors

  • André Luís Lopes da Silva Universidade Federal do Paraná
  • Yohana de Oliveira
  • Jefferson da Luz Costa
  • Elisângela Masetto
  • Eduardo Andrea Lemus Erasmo
  • Gessiel Newton Scheidt
  • André Luís Lopes da Silva Universidade Federal do Paraná
  • Yohana de Oliveira Universidade Federal do Paraná
  • Jefferson da Luz Costa Universidade Federal do Paraná https://orcid.org/0000-0002-8912-8208
  • Elisângela Masetto Universidade Federal do Paraná
  • Clarissa de Souza Mudry Universidade Federal do Paraná
  • Eduardo Andrea Lemus Erasmo Universidade Federal do Tocantins
  • Gessiel Newton Scheidt Universidade Federal do Tocantins https://orcid.org/0000-0002-4729-3416

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v1n1.silva

Keywords:

Eucalypt, woody plant, genetic transformation

Abstract

An efficient selec tive agent helps to obtain transgenic plant s; the most used selective agent for genetic transformation of Eucalypt is kanamycin. The aim of this research was to determine ideal dose of kanamycin in shoot tip and cotyledon explants of E. saligna for use as selective agent in genetic transformation. Two experiments were carried out, first in order to test effects of the kanamycin in cotyledons and second, in order to test effects of the kanamycin in shoot tip. In the cotyledons experiment were tested 0, 12.5, 25, 37.5 and 50 mg.L-1 kanamycin on a callus formation medium and for shoot tip were tested 50, 75, 100, 125 and 150 mg.L-1 kanamycin on a multiplication medium. Cotyledons can be selected more quickly than shoot tip, moreover needs lower kanamycin levels than shoot tip, what results more speed and economy during genetic transformation process. It is probable that levels lower than 12.5 mg.L-1 kanamycin must be ideal for the cotyledons selection and 50 mg.L-1kanamycin for shoot tip.

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Published

2010-11-08

How to Cite

da Silva, A. L. L., de Oliveira, Y., Costa, J. da L., Masetto, E., Lemus Erasmo, E. A., Scheidt, G. N., … Scheidt, G. N. (2010). Shoot tip and cotyledon explants of Eucalyptus saligna Sm. cultivated on different kanamycin levels. Journal of Biotechnology and Biodiversity, 1(1), 1–5. https://doi.org/10.20873/jbb.uft.cemaf.v1n1.silva

Issue

Vol. 1 No. 1 (2010): Journal of Biotechnology and Biodiversity

Section

Biotechnology

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