Effect of diazotrophic bacteria in the production components of sugarcane infected with ratoon stunting disease
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v2n4.souzaKeywords:
Saccharum spp, Herbaspirillum seropediceae, Azospirillum brasiliense, endophytic bacteriaAbstract
The aim of this study was to evaluate the effects of diazotrophic bacteria in the yield components of sugarcane infected with the ratoon stunting disease caused by Leifsonia xyli subsp. xyli. The test was conducted at the Experimental Station Paranavaí, PR, 2010. The treatments consisted of genotype SP77-5181 inoculated with: 1. Azospirillum brasiliense strain AbV5 + Leifsonia xyli subsp. xyli; 2. Herbaspirillum seropedicae strain SMR1 + Leifsonia xyli subsp. xyli; 3. Herbaspirillum seropedicae strain HCC103 + Leifsonia xyli subsp. xyli; 4. Leifsonia xyli subsp. xyli; 5. All bacteria (Azospirillum brasiliense + Herbaspirillum seropedicae + Leifsonia xyli subsp. xyli), 6. No inoculated treatment. Evaluations were made of: POL, tons of POL per hectare, fiber content, weight of a culm, number of stems per meter, height, stalk diameter and tons of cane per hectare for each treatment. The detection of the pathogen was analyzed by Dot blot. The design was a randomized block design with three replications. It was found that Herbaspirillumn seropedicae SMR1 strain, showed potential as biological control of colonization Leifsonia xyli subsp. xyli, based on evidence such as increasing the mass of stalks. Apparently the bacteria Azospirillum brasiliense strain: AbV5 caused no detectable effects on yield components evaluated.
References
Capone, A.; Lui1, J. J.; Silva1, T. R.; Dias, M. A. R.; Melo, A. V. (2011), Avaliação do comportamento de quinze cultivares de cana-de- açucar na Região Sul do Tocantins. Journal of Biotechnology and Biodiversity, 3, 70-78.
Carneiro Jr., J. B.; Silveira, F. S.; De Ponte, E. (2006), Sanidade e vigor de mudas de cana-de- açúcar infectadas por Leifsonia xyli subsp. xyli e tratadas por termoterapia. Fitopatologia Brasileira, 31, 183, suplemento.
Carneiro Jr., J. B. Obtenção e análise de especificidade de antissoro (policlonal) contra Leifsonia xyli subsp. xyli, agente causal do RSD. Campo dos Goytacazes. Dissertação (mestrado em fitopatologia) - Centro de Ciências Tecnológicas, Universidade Estadual do Norte Fluminense, 2001.
Costa, J. M. T. e Ruschel, A. P. (1981), Seasonal variation in the microbial populations of sugar- cane plants. In:VOSE, P. B., RUSCHEL, A. P.(Eds) CRC,109-118.
Damman, K. E. Jr. e Olliver, C. A. (1991), Distribution and incidence of ratoon stunting disease in Louisiana sugarcane. Plant disease,75, 568-571.
Döbereiner, J.; Baldani, V. L. D.; Reis, V. M. Endophytic occurrence of diazotrophic in non- leguminous crops. In: Azospirillum VI and related microorganisms (1995) (Ed: I. FENDRIK. et al.). Springer - Verlag, Berlin, Heidelberg, New York, 3-14.
Döbereiner, J. e Pedrosa, F. O. (1987) Nitrogen- fixing bacteria in nonleguminous crop plants. Science Tech Publishers/Springer-Verlag.
EMBRAPA, Centro Nacional de Pesquisa de Solos. Sistema brasileiro de classificação de solos (1999), Brasília, 412p.
Ferreira, D. F. Análises estatísticas por meio do Sisvar para Windows 4.0. (2000) In: Reunião anual da região brasileira da sociedade internacional de biometria, São Carlos: UFSCAR, 45, 255-258.
Harrison, N. A. e Davis, M. J. (1988), Colonization of vascular tissues by Clavibacter xyli subsp. xyli in stalks of sugarcane cultivars differing in susceptibility to roatoon stunting disease. Phytopathology, 78, 722-727.
INSTITUTO AGRONÔMICO DO PARANÁ. Cartas Climáticas do Estado do Paraná 1994 (1994), Londrina, 49 p. (IAPAR. Documento, 18).
Lee, T. S. G. (1988), Produção de mudas sadias de cana-de-açúcar através da técnica de cultura de tecidos. Álcool e Açúcar, Piracicaba, 45, 20-29.
Liu, L.; Kloepper, J. W.; Tuzun, S. (1995), Induction of systemic resistance in cucumber against fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology, 85, 695-698.
Lopes, A. A. C.; Reis Junior, F. B. (2008), Efeito com a inoculação com Herbaspirillum seropedicae sobre a produtividade do milho nos períodos de safra e safrinha. Brasília. Anais. IX Simpósio Nacional Cerrado. II Simpósio Internacional Savanas Tropicais.
Okon, Y.; Vanderleyden, J. (1997), Root- associated Azospirillum species can stimulate plants. American Society for Microbiology News, 63, 366–370.
Olivares, F. L.; Reis, V. M. e Façanha, A. R. (2000), The role of endophytic diazotrops in sugarcane rootmorphogenesis and development. In: Finan, T. M.; O'brian, M. R.; Layzell, D. B., Vessey, J. K. & Newton. W., eds. Nitrogen fixation: Global perspectives. Oxon, CAB International, 476-477.
Olivares, F. L. Taxonomia, ecologia e mecanismos envolvidos na infecção e colonização de cana-de- açúcar (Saccharum sp. híbrido) por bactérias diazotróficas endofíticas do gênero Herbaspirillum. Tese (Doutorado) - Universidade Federal Rural do Rio de Janeiro, Seropédica. 1997.
Pedrosa, F. O.; Yates, M. G. (1984), Regulation of nitrogen fixation (nif) genes of Azospirillum brasilense by nifA and ntrC (glnG) type genes. FEMS Microbiology Letters, 55: 95-101
Silva, H. S. A. Romeiro, R. S.; Macagnan, D.; Halfeld-Vieira, B. A.; Baracat-Pereira, M. C.; Mounteer, A. (2004), Rhizobacterial induction of systemic resistance in tomato plants: non-specific protection increase in enzyme activities. Biological Control, 29, 288-295.
Teakle, D. S.; Appleton, J. M.; Steindl, D. R. (1978), An anatomical basis for resistance of sugarcane to ratoon stunting disease. Physiol. Plant Pathology, 12, 83-91.
Tokeshi, H. Doenças da cana-de-açúcar (híbridos de Saccharum spp.) In: KIMATI, H. et al. Manual de Fitopatologia, 2: Doenças das Plantas Cultivadas. (1997), Editora Agronômica Ceres, São Paulo.
Xavier, R. P. Contribuição da fixação biológica de nitrogênio na produção sustentável da cultura de cana-de-acucar. Tese (Doutorado) Universidade Federal Rural do Rio de Janeiro, Seropédica-RJ, 2006.
Young, J. M.; Petrasovits, L. A.; Croft, B. J.; Gillings, M.; Brumbley, S. M. (2006), Genetic Uniformity of international isolates of Leifsonia xyli subsp. xily, causal agent of ratoon stunting disease of sugarcane. Australasian Plant Pathology, 35, 503-511.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 - Journal of Biotechnology and Biodiversity
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0 at http://creativecommons.org/licenses/by/4.0/) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Available at The Effect of Open Access, at http://opcit.eprints.org/oacitation-biblio.html).
