Potencial antioxidante da seiva do caule da planta Croton lechleri Müll. Arg. (Euphorbiaceae)
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v12n1.17403Keywords:
experimental design, extraction, blood dragon, total phenolic compoundsAbstract
The Croton lechleri plant is used by the indigenous and riverside population for its healing and anesthetic power. The objective of this research was to evaluate the amount of total phenolic compounds and the antioxidant properties of the sap extracted from the stem of the Blood Dragon (Croton lechleri) to use the natural extract as a renewable alternative to the synthetic antioxidants present in the food and biodiesel industry. The extraction conditions were optimized and the antioxidant activity of these bioactive compounds through fractional factorial design. The variations were: solvent (pure water, 50% ethanol, 100% ethanol), temperature (50 °C, 70 °C and 90 °C) and time (30 min, 60 min, 90 min). Nine assays were evaluated with the software Statist 7®. The total phenolic compounds content ranged from 92.22 ± 1.35 to 193.29 ± 1.93 mg of EAG/g of sap and antioxidant activity by the ABTS method from 15.17 ± 0.08 to 26.80 ± 0.04 mM TE/g sap. The analysis of the global response and its equations made it possible to verify that ethanol 50% (v/v) was more effective in extracting total phenolic compounds and antioxidant activity under conditions of 90°C and shorter extraction time, 30 minutes.
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