Enzymatic diversity of fungi associated with Caatinga plants
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v11n1.silvaKeywords:
semi-arid diversity , proteases, amylases, pectinasesAbstract
Filamentous fungi participate in food production, health products and recycling of biosphere com-pounds. In industry they are used in the production of enzymes, vitamins, polysaccharides, pigments, lipids and glycolipids, valuable in biotechnology. Brazil has a great biological diversity of landscapes and among them, the Caatinga, is widely explored regarding the plant's biotechnological potential, but there are few studies on the potential of microorganisms associated with this biome. This study aimed to evaluate the production of enzymes from fungi isolated in soil associated with plants from the semi-arid region. Soil samples were collected in the Grota do Angico region, municipality of Poço Redon-do, Sergipe, associated with two characteristic plants of the caatinga, Croton heliotropiifolius Kunth and Xiquexique gounellei. Thirty-nine fungi were isolated from soil samples and their proteolytic, amylolytic and pectinolytic activities were evaluated. The 17 fungi associated with C. heliotropiifolius present predominantly protease activity, only one of them showed pectinase activity, in the others the results were negative. On the other hand, the fungi 22 isolated from the soil associated with X. goiunellei showed greater enzymatic diversity, despite the fact that most fungi showed proteolytic activi-ty. Amylase activity was detected in eight fungi, protease activity in 12 fungi and pectinase activity in 6 fungi. From the results obtained in this study, the potential of fungi isolated from the caatinga biome for the production of enzymes, mainly proteolytic, was evidenced. Fungi isolated from soil associated with Caatinga plants have potential for the production of enzymes, mainly proteolytic ones.
References
Abreu JAS, Rovida AFS, Pamphile JA. Fungos de interesse: aplicações biotecnológicas. Revista Uningá Review, v.21, n.1, p.55-59, 2015.
Aguilar JGS, Sato HH. Microbial proteases: Production and application in obtaining protein hydrolysates. Food Research International, v.103, p.253-262, 2018. https://doi.org/10.1016/j.foodres.2017.10.044
Almeida AS, Santos AF. Potencial anticolinesterásico de plantas do bioma Caatinga: uma revisão. Diversitas Jour-nal, v.3, n.2, p.505-518, 2018. https://doi.org/10.17648/diversitas-journal-v3i2.589
Andrade ICB, Souza HTR, Santos LJP, Cruz IS, Gois DV, Souza RM. Relação clima-vegetação no monumento natural Grota do Angico- Sergipe-Brasil. Interfaces Científicas - Saúde e Ambiente, v.4, n.1, p.71, 2015. https://doi.org/10.17564/2316-3798.2015v4n1p71-82
Bald DRQ, Rangel CP, Vargas AF, Girão KT, Passaglia L. M. P. Microbiota do solo: a diversidade invisível e a sua importância. Revista Bio Diverso, v.1, p.101-31, 2021.
Belinato JR, Baziolia JM, Sussulini AAF, Fill TP. Metabolô-mica microbiana: inovações e aplicações. Química Nova, v.42, n.5, p.546-559, 2019. https://doi.org/10.21577/0100-4042.20170324
Cavalcanti DFG, Silveira DM, Silva GC. Aspectos e poten-cialidades biológicas do gênero Croton (Euphorbiaceae)/ Bi-ological aspects and potentialities of the genus Croton (Eu-phorbiaceae). Brazilian Journal of Development, v.6, n.7, p.45931-45946, 2020. https://doi.org/10.34117/bjdv6n7-280
Costa CZ, Albuquerque MCC, Brum MC, Castro AM. De-gradação microbiológica e enzimática de polímeros: uma re-visão. Química Nova, v.38, n.2, 2015. https://doi.org/10.5935/0100-4042.20140293
Esiana BOI, Coates CJ, Adderley WP, Berns AE, Bol R. Phenoloxidase activity and organic carbon dynamics in his-toric Anthrosols in Scotland, UK. PLoS ONE, v.16, n.10, p.1-18. https://doi.org/10.1371/journal.pone.0259205
Far BE, Ahmadi Y, Khosroshahi AY, Dilmaghani A. Micro-bial alpha-amylase production: progress, challenges and per-spectives. Advanced Pharmaceutical Bulletin, v.10, n.3, p.350-358, 2020. https://doi.org/10.34172/apb.2020.043
Ferreira EPB, Stone LF, Martin-Didonet CCG. População e atividade microbiana do solo em sistema agroecológico de produção. Revista Ciência Agronômica, v.48, n.1, p.22-31, 2017. https://doi.org/10.5935/1806-6690.20170003
Filizola HF, Gomes MAF, Souza MD. Manual de procedi-mentos de coleta de amostras em áreas agrícolas para análise da qualidade ambiental: solo, água e sedimentos. Jaguariúna: Embrapa Meio Ambiente. 169 p., 2006. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129660/1/2006OL-008.pdf. Acesso em: 03 out. 2022.
Fraga ME, Braz, DM, Rocha JF, Pereira MG, Figueiredo DV. Interação microrganismo, solo e flora como condutores da diversidade na Mata Atlântica. Acta Botanica Brasileira, v.26, n.4, p.857-865, 2012. https://doi.org/10.1590/S0102-33062012000400015
González, JÁ; Gallardo, CS; Pombar, A; Rego, P; Rodríguez, LA. Determination of enzymatic activities in ecotypic Sac-charomyces and non-Saccharomyces yeasts. Electronic Journal Environmental, Agricultural and Food Chemistry, v.3, n.5, p.743-750, 2004.
Hagerman AE, Blau DM, McClure AL. Plate assay for deter-mining the time of production of protease, cellulase, and pectinases by germinating fungal spores. Analytical Bio-chemistry, v.151, n.2, p.334-342, 1985. https://doi.org/10.1016/0003-2697(85)90184-8
Haile S, Ayele A. Pectinase from microorganisms and its industrial applications. Scientific World Journal. v.2022, p.1-15, 2022. https://doi.org/10.1155/2022/1881305
Kumar A, Chandra R. Ligninolytic enzymes and its mecha-nisms for degradation of lignocellulosic waste in environ-ment. Heliyon., v.6, n.2, p.1-18, 2020. https://doi.org/10.1016/j.heliyon.2020.e03170
Leal MLA, Chaves JS, Silva JA, Silva LS, Soares RB, Nas-cimento JPS, Matos SM, Texeira Junior DL, Brito Neto AF. Efeito dos sistemas de manejo e do uso do solo na pop-ulação de microrganismos do solo. Research, Society and Development, v.10, n.9, p.1-11, 2021. https://doi.org/10.33448/rsd-v10i9.17966
Marroques, JC. Aplicação de enzimas na indústria têxtil. Ano de obtenção: 2020, 47 p. Trabalho de Conclusão de Curso (Bacharelado em Engenharia Química) - Universidade Fede-ral de Uberlândia, Uberlândia.
Mendoza AYG, Silva VV, Lima RA, Lima JPS. Potenciali-dades biotecnológicas dos fungos da amazônia brasileira: uma revisão sistemática. Diversitas Journal, v.7, n.4, p.2535-2551 2022. https://doi.org/10.48017/dj.v7i4.2104
Menezes EHA, Silva AIB, Costa AEN, Mendonça CMS, Nunes LE, Oliveira LC, Cavalcante JS, Sá-Filho GF, Costa AEN. As plantas do bioma caatinga com potencial neuro-protetor: uma revisão integrativa. Revista Biodiversidade, v.20, n.4, p.121-139, 2021.
Mesquita MOM, Pinto TMF, Moreira RF. Potencial antimi-crobiano de extratos e moléculas isolados de plantas da Caatinga: uma revisão. Revista Fitos, v.11, n.2, p.119-249, 2017. https://doi.org/10.5935/2446-4775.20170028
Monteiro VN, Silva RN. Aplicações industriais da biotecnolo-gia enzimática. Revista Processos Químicos, v.3, p.9-23, 2009. https://doi.org/10.19142/rpq.v3i5.83
Montoya-Castrillón M, Serna-Vasco KJ, Pinilla L, Rico JMQ, Cardona-Bermúdez LM, Osorio-Echavarria. Isolation and characterization of filamentous fungi from wood and soil samples of “La Lo-rena”, Sonsón, Antioquia (Colombia), natural reserve. DYNA, v.88, n.219, p.171-180, 2021. https://doi.org/10.15446/dyna.v88n219.96144
Motato-Vásques V, Pires RM, Vitali VMV, Gugliotta ADM. Cultural and ligninolytic activity studies of some polypores (Basidiomycota) from brazilian Atlantic Forest, São Paulo, Brazil. Hoehnea, v.43, n.2, p.289-300, 2016. https://doi.org/10.1590/2236-8906-81/2015
Pessoa VG, Santos SCL, Oliveira PVC, Silva TGP, Oliveira PVC, Fernandes GST. Bromatological composition and sensory analysis of cacti as unconventional food plants: a systematic review. Research, Society and Development, v.11, n.8, p.1-11, 2022. https://doi.org/10.33448/rsd-v11i8.31289
Peraza-Jiménez K, De la Rosa-García S, Huijara-Vasconselos JJ, Reyes-Estebanez M, Gómez-Cornelio S. Enzymatic Bio-prospecting of Fungi Isolated from a Tropical Rainforest in Mexico. Journal of Fungi, v.8, n.1, p.1-15, 2022. https://doi.org/10.3390/jof8010022
Razzaq A, Shamsi S, Ali A, Ali Q, Sajjad M, Malik A, Ashraf M. Microbial Proteases Applications. Frontiers in Bioengi-neering and Biotechnology, v. 7, p.1-20, 2019. https://doi.org/10.3389/fbioe.2019.00110
Saccaro Junior NL. A regulamentação de acesso a recursos genéticos e repartição de benefícios: disputas dentro e fora do Brasil. Ambiente & Sociedade, v.16, p.229-244, 2011. https://doi.org/10.1590/S1414-753X2011000100013
Samuel P, Maheswari M, Vijayakumar J, Selvarathinam T, Amirtharaj K, Deenathayalan R. Bio-prospecting of marine-derived fungi with special reference to production of ecovaluable enzyme keratinase - A need-based optimization study. Journal of Applied Biology and Biotechnology, v.6, n.3, p.35-41, 2018. https://doi.org/10.7324/JABB.2018.60306
Santana I. Mistura de enzimas mostrou alto desempenho para gerar etanol a partir de bagaço de cana. Embrapa Notícias, 27 maio, 2020. Disponível: https://www.embrapa.br/busca-de-noticias/-/noticia/52639673/mistura-de-enzimas-mostrou-alto-desempenho-para-gerar-etanol-a-partir-de-bagaco-de-cana/. Acesso em: 15 jan. 2023.
Sidrim JJC, Rocha MFG. Micologia Médica à luz de autores contemporâneos. Rio de Janeiro: Guanabara Koogan, 2004.
Silva LL, Moreira IC, Silva MT, Silva BL, Santos AAT, Oliveira ML, Souza DF, Brito AO, Santos SX. Biopros-pecção de Fungos de um Frag-mento do Cerrado no Brasil Central para Aplicações Biotec-nológicas. Fronteiras: Jour-nal of Social, Technological and Environmental Science, v.7, n.1, p.288-305, 2018. https://doi.org/10.21664/2238-8869.2018v7i1.p288-305
Silva CJA, Malta DJN. A importância dos fungos na biotec-nologia. Cadernos de Graduação, v.2, n.3, p.49-66, 2017.
Silva JAG, Lima IR, Santana MAN, Silva TMS, Silva MIAG, Leite SP. Screening Fitoquímico e Avaliação da Toxicidade de Croton heliotropiifolius Kunth (Euphorbiaceae) frente à Artemia salina Leach. Rev. Virtual Quimica, v.9, n.3, p.934-941, 2017. https://doi.org/10.21577/1984-6835.20170060
Silva JBA, Frantz SC, Marques AK, Coelho CM, Morais PB. Produção de enzimas extracelulares por fungos associados à decomposição de materiais de vegetais em riachos. Journal of Bioenergy and Food Science, v.4, n.2, p.2008-212, 2015. https://doi.org/10.18067/jbfs.v2i4.75
Silva ACC, Prata APN, Souto LS, Mello, AA. Aspectos de ecologia de paisagem e ameaças à biodiversidade em uma unidade de conservação na Caatinga, em Sergipe. Revista Ár-vore, v.37, n.3, 2013. https://doi.org/10.1590/S0100-67622013000300011
Silva DCV, Tiago PV, Mattos JLS, Paiva LM, Souza-Motta CM. Isolamento e seleção de fungos filamentosos do solo de sistemas agroflorestais do Município de Bom Jardim (PE) com base na capacidade de produção de enzimas hidro-líticas. Revista Brasileira de Botanica, v.34, n.4, p.607-610, 2011. https://doi.org/10.1590/S0100-84042011000400014
Silva JGM, Silva, DS, Ferreira MA, Lima GFC, Melo AAS, Diniz MCNM. Xiquexique (Pilosocereus gounellei (A. We-ber ex K. Schum.) Bly. ex Rowl.) em Substituição à Silagem de Sorgo (Sorghum bicolor L. Moench) na Alimentação de Vacas Leiteiras. Revista Brasileira de Zootecnia, v.34, n.4, p.1408-1417, 2005. https://doi.org/10.1590/S1516-35982005000400039
Souza FR. Aplicação de enzimas no processamento de couros: comparação entre processos químicos e enzimáticos. Ano de obtenção: 2010, 144 p. Dissertação de Mestrado (Engenha-ria Química) - Universidade Federal do Rio Grande do Sul, Porto Alegre.
Valencia JWA. Metabólitos de origem fúngica: aplicações potenciais em processos biotecnológicos. 2011, 117p. Tese de doutorado (Biologia Molecular), Universidade de Brasí-lia, Brasília.
Wetler-Tonini RMC, Rezende CE, Grativol AD. Degradação e biorremediação de compostos do petróleo por bactérias: re-visão. Oecologia Australis, v.14, n.4, p.1010-1020, 2010. https://doi.org/10.4257/oeco.2010.1404
Zhang, X., Shuai Y, Tao H, Li C, He L. Novel Method for the Quantitative Analysis of Protease Activity: The Casein Plate Method and Its Applications. ACS Omega, v.6 n.5, p.3675-3680, 2021. https://doi.org/10.1021/acsomega.0c05192
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