Physicochemical evaluation and biological activities of the extract uvaia (Eugenia pyriformis Camb.)

Abstract

The Myrtaceae family has numerous fruit genera such as Eugenia. The aim of this work was to evaluate the hydroethanolic extract of the ripe fruit of Eugenia pyriformis on the physicochemical characteristics and photoprotection and cytotoxic on human erythrocytes. The ripe fruits were collected and pulped. The extract was produced by maceration in a 70% hydroethanolic solution. The physical-chemical characterization was performed for qualitative sensory analysis, extract yield, dry mass, water content, pH, total solids, refractive index, relative density, total flavonoid and tannin contents, thin layer chromatography, scanning between 400 at 900 nm, photoprotection scanning activity between 220 to 400 nm and hemolytic activity on human erythrocytes and different concentrations of extract. The results showed yellow, homogeneous and aromatic color for the extract. Yield of 20.05%, dry mass 16.18%, water content 83.82%, pH 2.64, total solids 2.07%, refractive index 1.4025 n_D, density 1.013 g mL^-1, flavonoids 72.87 mg RU 100 g^-1, tannins 6.05 mg CA 100 g^-1, several Rfs, with the acetone/chloroform elotropic set having a greater number of isolated compounds, presence of natural sugars glucose and sucrose. UVC and cytotoxic photoprotection, activity on erythrocytes between 35.04% to 2.14%. The results showed safety on the use of the hydroethanolic extract of the fruit of E. pyriformis being able to be used as a photoprotective solution of the natural line. Further studies should be carried out evaluating other biological activities by extracting the fruit of Eugenia pyriformis.