Evaluation of green microalgae isolated from central and north coast of Sao Paulo as source of oil
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v9n1.matsudoPalabras clave:
microalgae, biomass, single-cell-oil, fatty acids, lipidsResumen
Microalgae strains, newly isolated from freshwater in mangrove areas of Central and North Coasts of Sao Paulo State (Brazil), were evaluated regarding total protein and lipid content, and fatty acids profile. The biochemical composition was compared with that observed in strains obtained by UTEX Culture Collection (USA). Among seven identified green algae, Monoraphidium contortum (CCMA-UFSCar-701) presented the highest lipid content (43.60%), close to that observed in Botryococcus braunii (UTEX-2441; 48.85%). Protein content in isolated strains varied in the range of 13.90~23.60%. Finally, the most abundant fatty acids were palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), and y-linolenic acid (C18:3).Chlorella vulgaris (CCMA- UFSCar-704) may be highlighted for its high linoleic acid content (49%). On the other hand, Elakatothrix sp (CCMA- UFSCar-702) and Scenedesmus obliquus (UTEX-B2630) presented the highest content of oleic acid (41% and 43%, respectively), which is preferable for oils to be used as feedstock for biodiesel.
Citas
Andersen RA, Kawachi M. Traditional micoalgae isolation techniques. In: Andersen RA (Ed.) Algal culturing tech-niques. Esevier Academic Press, pp.83-100, 2005
AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists, 14th ed. Arlington: American Public Health Association, 1141p. 1984.
Avila-Leon I, Matsudo MC, Sato S, Carvalho JCM. Arthro-spira platensis biomass with high protein content cultivated in continuous process using urea as nitrogen source. Journal of Applied Microbiology, v. 112, p. 1086–1094, 2012. https://doi.org/10.1111/j.1365-2672.2012.05303.x
Barka A, Blecker C. Microalgae as a potential source of sin-gle-cell proteins. A review. Biotechnology, Agronomy, So-ciety and Envionment, v. 20, p. 427–436, 2016. DOI: 10.25518/1780-4507.13132
Becker EW. Microalgae - Biotechnology and Microbiology. New york: Cambridge University Press, 293p. 1994.
Beijerinck MW. Kulturversuche mit Zoochlorellen, Lichenen-gonidien und anderen nie-deren Algen. Bot Zeitung, v. 47, p. 725–788, 1980.
Bellou S, Baeshen MN, Elazzazy AM, Aggeli D, Saygh F, Aggelis G. Microalgal lipids biochemistry and biotechno-logical perspectives. Biotechnology Advances, v.32, p. 1476–1493, 2014. https://doi.org/10.1016/j.biotechadv.2014.10.003
Brown ML, Zeiler KG. Aquatic biomass and carbon dioxide trapping. Energy Conversion and Management, v.34, p. 1005-1013, 1993. https://doi.org/10.1016/0196-8904(93)90048-F
Chen X, He G, Deng Z, Wang N, Jiang W, Chen S. Screening of Microalgae for Biodiesel Feedstock. Advances in Micro-biology,v.4, p.365-376, 2014. https://doi.org/10.4236/aim.2014.47044
Chisti Y. Biodiesel from microalgae. Biotechnology Advanc-es, v.25, p.294-306, 2007. https://doi.org/10.1016/j.biotechadv.2007.02.001
Chisti Y. Microalgae: our marine forests. In: Richmond A. (Ed.) Handbook of microalgal culture: biotechnology and applied phycology. Oxford: Blackwell Science. 566p. 2004.
Cruz-Martínez LC, Jesus CKC, Matsudo MC, Danesi EDG, Sato S, Carvalho JCM. Growth and composition of Arthro-spira (Spirulina) platensis in a tubular photobioreactor using ammonium nitrate as the nitrogen source in a fed-batch pro-cess. Brazilian Journal of Chemical Engineering, v.32, p.347-356, 2015. https://doi.org/10.1590/0104-6632.20150322s00003062
Derner RB, Ohse S, Villela M, Villela M, Carvalho SM, Fett R. Microalgae, products and applications. Ciência Rural, v.36, p.1959-1967, 2006. https://doi.org/10.1590/S0103-84782006000600050
Dunstan GA, Volkman JK, Barrett SM, Garland CD. Changes in the lipid composition and maximisation of the polyunsatu-rated fatty acid content of three microalgae grown in mass culture. Journal of Applied Phycology, v.5, p.71-83, 1993. https://doi.org/10.1007/BF02182424
Gouveia L, Marques AE, Sousa JM, Moura P, Bandarra N. Microalgae – source of natural bioactive molecules as func-tional ingredients. Food Science and Technology Bulletin, v.7, p. 21-37, 2011. https://doi.org/10.1616/1476-2137.15884
Guillard RRL, Ryther JH. Studies of marine planktonic dia-toms. Canadian Journal of Microbiology, v.8, p.229-239, 1962. https://doi.org/10.1139/m62-029
Hartman L, Lago RC. Rapid preparation of fatty acid methyl esters from lipids. Laboratory Practices, v.22, p.475-477, 1973.
Horrobin DF. Nutritional and medical importance of gamma-linolenic acid. Progress in Lipid Research, v.31, p.163-194, 1992. https://doi.org/10.1016/0163-7827(92)90008-7
Janssen M, Tramper J, Mur LR, Wijffels RH. Enclosed out-door photobioreactors: Light regime, photosynthetic effi-ciency, scale-up, and future prospects. Biotechnology and Bioengineering, v.81, p.193-210, 2003. https://doi.org/10.1002/bit.10468
Knothe G. “Designer” Biodiesel: optimizing fatty ester compo-sition to improve fuel properties. Energy Fuels, v.22, p.1358-1364, 2008. https://doi.org/10.1021/ef700639e
Komárková-Legnerová J. The systematics and ontogenesis of the genera Ankistrodesmus Corda and Monoraphidium gen. nov. Academia, 70p. 1969.
Kus MMM, Silva SA, Aued-Pimentel S, Mancini-Filho J. Nutrition facts of infant formulas sold in São Paulo state: assessment of fat and fatty acid contents. Revista de Nu-trição, v.24, p.209-218, 2011. https://doi.org/10.1590/S1415-52732011000200002
Li Y, Han D, Hu G, Sommerfeld M, Hu Q. Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii. Biotechnology and Bioengi-neering, v.107, p.258-268, 2010. https://doi.org/10.1177/0969733010361439
Liu B, Benning C. Lipid metabolism in microalgae distin-guishes itself. Current Opinion in Biotechnology, v.24, p.300-309, 2013. https://doi.org/10.1016/j.copbio.2012.08.008
Mahmoud EA, Farahat LA, Abdel Aziz ZK, Fatthallah NA, El Din RAS. Evaluation of the potential for some isolated mi-croalgae to produce biodiesel. Egyptian Journal of Petrole-um, v.24, p.97-101, 2015. https://doi.org/10.1016/j.ejpe.2015.02.010
Matsudo M, Bezerra R, Sato S, Perego P, Converti A, Carva-lho JCM. Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source. Bio-chemical Engineering Journal, v.43, p.52-57, 2009. https://doi.org/10.1016/j.bej.2008.08.009
Matsudo MC, Bezerra RP, Sato S, Converti A, Carvalho JCM. Photosynthetic efficiency and CO2 assimilation rate of Arthrospira (Spirulina) platensis continuously cultivated in tubular photobioreactor. Biotechnology Journal, v.7, p.1412-1417, 2012. https://doi.org/10.1002/biot.201200177
Molino A, Iovine A, Casella P, Mehariya S, Chianese S, Cerbone A, Rimauro J, Musmarra D. Microalgae characteri-zation for consolidated and new application in human food, animal feed and nutraceuticals. International Journal of Envi-ronmental Research and Public Health, v.15, p.1-21, 2018. https://doi.org/10.3390/ijerph15112436
Norton TA, Melkonian M, Andersen RA. algal biodiversity. Phycologia, v.35, p.308-326, 1996. https://doi.org/10.2216/i0031-8884-35-4-308.1
Olaizola M. Commercial development of microalgal biotech-nology: From the test tube to the marketplace. Biomolecular Engineering, v. 20, p. 459–466, 2003. https://doi.org/10.1016/S1389-0344(03)00076-5
Olguín E, Galicia S, Angulo O, Hernández E. The effect of low light flux and nitrogen deficiency on the chemical com-position of Spirulina sp. growth on pig waste. Bioresourse Technology, v.77, p.19-24, 2001. https://doi.org/10.1016/S0960-8524(00)00142-5
Pérez-Mora LS, Matsudo MC, Cezare-Gomes EA, Carvalho JCM, An investigation into producing Botryococcus braunii in a tubular photobioreactor. Journal of Chemical Technol-ogy and Biotechnology, v.91, p.3053-3060, 2016. https://doi.org/10.1002/jctb.4934
Piorreck M, Baasch K, Pohl P. Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry, v.23, p.207-216, 1984.
Pulz O, Gross W. Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol, v.65, p.635-648, 2004. https://doi.org/10.1007/s00253-004-1647-x
Ract JNR, Gioielli LA. Modified lipids obtained from milk fat, sunflower oil, and phytosterols esters for application in spreads. Quim Nova, v.31, p.1960-1965, 2008. https://doi.org/http://dx.doi.org/10.1590/S0100-40422008000800008
Ramazanov A, Ramazanov Z. Isolation and characterization of a starchless mutant of Chlorella pyrenoidosa STL-PI with a high growth rate, and high protein and polyunsaturated fatty acid content. Phycological Research, v.54, p.255-259, 2006. https://doi.org/10.1111/j.1440-1835.2006.00416.x
Ronda SR, Lele SS. Culture conditions stimulating high γ-linolenic acid accumulation by Spirulina platensis. Brazilian Journal of Microbiology, v.39, p.693-697, 2008. https://doi.org/10.1590/S1517-83822008000400018
Sassano CEN, Gioielli LA, Ferreira LS, Rodrigues MS, Sato S, Converti A, Carvalho JCM. Evaluation of the composi-tion of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source. Bi-omass and Bioenergy, v.34, p.1732-1738, 2010. https://doi.org/10.1016/j.biombioe.2010.07.002
Schlösser UG. Sammlung von Algenkulturen. Berichte der Deutschen Botanischen Gesellschaft, v.95, p.181-276, 1982. https://doi.org/10.1111/j.1438-8677.1982.tb02862.x
UTEX. The Culture Collection of Algae at the University of Texas at Austin. http://www.sbs.utexas.edu/utex/. Accessed 3 Sep 2011
Verlengia R, Lima TM. Síntese de Ácidos Graxos. In: Curi R, Pompeia C, Miyasaka CK, Procópio J (Eds) Entendendo a gordura: os ácidos graxos. São Paulo: Manole. pp.121-134, 2002.
Wille N. Beschreibung einiger Planktonalgen aus norwe-gischen süsswasserseen. Biologisches Centralblatt, v.18, p. 302, 1898.
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