Different concentrations of naphthaleneacetic acid in the in vitro establishment of cassava
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v8n4.martinsKeywords:
micropropagation, Manihot esculenta, phytoregulatorsAbstract
Cassava culture has high socioeconomic relevance for Brazil. However, in the western region of Pará, the spread of pests and diseases, among other factors, has limited root production. In this way, micropropagation becomes an alternative to traditional cultivation methods for obtaining material with phytosanitary quality. The objective of this study was to evaluate the effect of different concentrations of naphthalene acetic acid (ANA) in the in vitro establishment of stem apexes of the cassava variety Água Morna. The stem apexes were introduced into MS medium, supplemented with 0.04 mg L‑1 of 6-benzylaminopurine, 0.05 mg L‑1 of gibberellic acid and different concentrations of ANA (0.00 mg L‑1; 0.01 mg L‑1; 0.02 mg L‑1 and 0.03 mg L‑1). The experimental design was completely randomized, with five treatments, containing five replicates of five tubes each. Over 30 days, the height of the seedlings, number of green and dead leaves, number of roots and the length of the largest root were evaluated. The seedlings showed average values of 1.51 cm in height, 1.51 green leaves, 1.67 roots and 1.31 cm in length from the largest root. The control treatment provided the least number of dead leaves, with an average of 0.52 leaves. There were no significant differences between ANA concentrations for the other variables analyzed, except for the number of dead leaves. Therefore, the in vitro establishment of the Água Morna cassava variety was efficient in all treatments, and the culture medium without growth regulators could be used.
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