Mini rose (Rosa sp.) nodal segments asepsis for in vitro establishment
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v7n1.silvaKeywords:
plant tissue culture, explants, sodium hypochlorite, ornamental plantsAbstract
Brazil stands out as one of the largest producers of ornamental plants in the world. The mini roses (Rosa sp.) are among the most commercialized vase flowers. However, despite of their great economic importance, traditional propagation methods presents undesirable characteristics, since they favor the spread of diseases and pests, seasonal dependence and low multiplication rate. Therefore, the objective of this study was to evaluate the mini rose nodal segments asepsis using different concentrations and immersion times in sodium hypochlorite solution (NaClO). Nodal segments were collected from young mini red roses plants, straight from the field. Nine treatments were evaluated, combining the concentrations of 0.75, 1.00 and 1.25% NaClO and the times of 10, 20 and 30 minutes of exposure the explants to disinfectant agent. The experimental design was completely randomized, with nine treatments containing 20 replications. Over 30 days of in vitro culture, the percentage of explant contamination, survival and necrosis were evaluated. According to the data obtained, there was a high contamination rate in all treatments. However, explants submitted to 1.25% NaClO concentration for 30 minutes had the highest aseptic rate (45%). Also, in this same treatment, no bacterial contamination or explant necrosis were observed. Therefore, the mini rose nodal segments submitted to sodium hypochlorite at 1.25% concentration for 30 minutes of immersion obtained the highest percentage of asepsis.
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