Virus associated with thickening of the cladodes of prickly pear (Opuntia ficus-indica Mill.)

Authors

  • Alba Suaste Dzul Instituto de Fitossanidad - Fitopatologia do México
  • Reyna Isabel Rojas Martinez Instituto de Fitossanidad - Fitopatologia del México
  • Daniel Ochoa Martínez Instituto de Fitossanidad - Fitopatologia del México
  • Emma Zavaleta Mejía Instituto de Fitosanidad - Fitopatología del México
  • Daisy Pérez Brito Universidad Antónoma de Chapingo
  • Camilo Hernández Juárez Universidad Autónoma de Chapingo
  • Douglas Rodríguez Martínez Colégio de Postgraduandos do Estado do México

DOI:

https://doi.org/10.20873/jbb.uft.cemaf.v2n4.dzul

Keywords:

dsRNA, long flexible particles, transmission eletron microscopy

Abstract

Symptoms putative to phytoplasmas and virus as yellowing, mosaic, proliferation and deformation of fruits and thickening of the cladodes of prickly pear have been observed in Nopaltepec, Estado de México. The objective of this investigation was to detect the presence of virus in samples of prickly pear showing thickening syndrome of the cladodes. The analysis of double-stranded RNA in prickly pear tissue whit the symptoms mentioned above revealed the presence of viral RNA. In symptomatic tissue, flexible rods of 950-1700 nm in length were observed with the aid of transmission electron microscopy. The virus was transmitted mechanically to Nicotiana tabacum var. Xanthi, N. glutinosa, N. occidentalis, N. benthamiana, Chenopodium quinoa, C. amaranticolor and Datura stramonium. Results from RT-PCR indicate that the particle does not correspond to TMV, neither to member of the Potyviridae family or species of the genus Potexvirus.   

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Published

2012-11-17

How to Cite

Alba, Reyna Isabel, Daniel, Emma, Daisy, Camilo, … Martínez, D. R. (2012). Virus associated with thickening of the cladodes of prickly pear (Opuntia ficus-indica Mill.). Journal of Biotechnology and Biodiversity, 3(4), 100–107. https://doi.org/10.20873/jbb.uft.cemaf.v2n4.dzul