Fusarium oxysporum f. sp. cubense biocontrol mediated by Pseudomonas spp. in Prata-Anã banana
Biocontrol of Fusarium oxysporum in banana by Pseudomonas
DOI:
https://doi.org/10.20873/jbb.uft.cemaf.v10n1.vieiraKeywords:
Biological Control, Pseudomonas spp, Panama diasease.Abstract
The banana tree is cultivated in the main countries of the world, including Brazil. This crop presents several phytosanitary problems, especially the Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubense (Foc). The use of microorganisms such as Pseudomonas has been an alternative for the biocontrol of phytopathogens. In order to investigate the control capacity of rhizobacteria of the genus Pseudomonas in relation to the activity of the fungus Fusarium oxysporum f. sp. cubense (Foc), two experiments were carried out under greenhouse conditions. The design was completely randomized (DIC) with three treatments, three controls and 10 replications. The treatments of the first experiment consisted of applying Pseudomonas spp. on the substrate, with the addition of Foc with and without a time interval. After inoculation period in the substrate infested by Foc and Pseudomonas, banana seedlings were planted in tubes. In the second experiment, the application interval was the same as in the pre-inoculation experiment, but Fusarium was inoculated first and then the bacteria and then transferred to the substrate. The use of Pseudomonas in different treatments, with time intervals, was not efficient to control Foc. It was observed that the disease index was less severe in the treatment without time interval for inoculation of Fusarium and Pseudomonas, being the best treatment for the biocontrol of Fusarium in banana plants of the cultivar Prata Anã. The use of bacteria of the genus Pseudomonas spp. is an economically viable alternative to control Fusarium Wilt.
References
Alzandi AA, Naguib DA. Metabólitos de Pseudomonas fluo-rescens como agente de biopriming para indução de resis-tência sistêmica em tomateiro contraFusariummurchar. Rhi-zosphere, v.11, p.124-128, 2019. https://doi.org/10.1016/j.rhisph.2019.100168
Anjum S, Sundaram S, Rai GK. Nutraceutical application and value addition of banana peel: A review. International Jour-nal of Pharmacy and Pharmaceutical Sciences, v.6, n.10, p.81-85, 2014.
Arya A, Gupta K, Chundawat TS, Vaya D. Biogenic Synthe-sis of Copper and Silver Nanoparticles Using Green Alga Botryococcus braunii and Its Antimicrobial Activity. Bio-inorganic Chemistry and Applications, v.2018, p.1-9, 2018. https://doi.org/10.1155/2018/7879403
Bholay AD, Jadhav UP, Borkhataria BV. Fluorescent pseu-domonads as plant growth promoting rhizobacteria and their siderophoregenesis. Journal of Pharmacy and Biological Sciences, v.3, p.27-32, 2012. https://doi.org/10.9790 / 3008-0312732
Borges AL, Souza LS, Oliveira AMG. Banana in: Crisósto-mo, L. A.; Naumov, A.(org.). Adubando para alta produti-vidade e qualidade: fruteiras tropicais do Brasil. Fortaleza: Embrapa Agroindústria Tropical, 238p., 2009. (IIP. Boletim 18).
Brandão LP, Souza CPF, Pereira VM, SILVA SO, Santos-Serejo JA, Ledo CAS, Amorim EP. Descriptor selection for banana accessions based on univariate and multivariate anal-ysis. Genetics and Molecular Research, v.12, n.2, p.1603-1620, 2013. http://dx.doi.org/10.4238/2013.May.14.1
Cordeiro ZJM, Shepherd K, Soares Filho WS, Dantas JLL. Avaliação de resistência ao mal-do-Panamá em híbridos te-traploides de bananeira. Fitopatologia Brasileira, Brasília, v.18, n.4, p.478-483, 1993.
Cordeiro ZJM; Doenças fúngicas e bacterianas In: Ferreira CF (org.) O agronegócio da banana. 1 ed. Brasília-DF, Embra-pa/ Cruz das Almas, p.547-575, 2016.
Corrêa EB, bettiol W, Sutton JC. Controle biológico da podri-dão radicular (Pythium aphanidermatum) e promoção de crescimento por Pseudomonas chlororaphis 63-28 e Bacillus subtilis GB03 em alface hidropônica. Summa Phytopatho-logica, v.36, n.4, p.275-281, 2010. https://doi.org/10.1590/S0100-54052010000400001
Durairaj K, Velmurugan P, Park JH, Chang WS, Park YJ, Senthilkumar P, Oh BT. An investigation of biocontrol ac-tivity Pseudomonas and Bacillus strains against Panax gin-seng root rot fungal phytopathogens. Biological control, 9 ed., v.125, p.138-146, 2018.
FAOSTAT (2021). Banco de dados online FAOSTAT. Dis-ponível em http://faostat.fao.org/ (acessado em janeiro de 2021).
Ferreira DF. Sisvar: a computerstatisticalanalysis system. Ciência e Agrotecnologia, Lavras, v.35, n.6, p.1039-1042, 2011.
Freitas SS. Rizobactérias promotoras do crescimento de plan-tas. In: SILVEIRA, A. P. D.; FREITAS, S. S. Microbiolo-gia do solo e qualidade ambiental. Campinas: Instituto Agronômico, 2007.
Gamez RM, Rodríguez F, Vidal NM, Ramirez S, Alvarez RV, Landsman D, Mariño-Ramirez L. Perfil do transcriptoma de banana (Musa acuminata) em resposta a rizobactérias: Baci-llus amyloliquefaciens Bs006 e Pseudomonas fluorescens Ps006. BMC Genomics, v.20, n.378, p.121-133, 2019. https://doi.org/10.1186/s12864-019-5763-5
Gonçalves zs, Haddad f, Amorim vbo, Ferreira cf, de Oliveira sa. s, Amorim ep. Agronomic characterization and identifi-cation of banana genotypes resistant to Fusarium wilt race 1. European Journal of Plant Pathology, v.155, p.1093-1103, 2019. https://doi.org/10.1007/s10658-019-01837-5
Huang Y, Hickman JE, Wu S. 2018: Impactos de aplicações aprimoradas de fertilizantes no ozônio troposférico e danos às colheitas na África Subsaariana. Atmosferic Environmen-tal, v.180, p.117-125, 2018. https://doi.org/10.1016/j.atmosenv.2018.02.040
IBGE. Banco de Dados Agregados. Sistema IBGE de Recupe-ração Automática –SIDRA.2015. Disponível em: <http://www2.sidra.ibge.gov.br/bda/tabela/listabl.asp?c=1613&z=t&o=11>.Acesso em: 27 nov. 2016.
Islam MT, Kim KH, Choi J. Wheat Blast in Bangladesh: The Current Situation and Future Impacts. The Plant Pathology Journal, v.35, n.1, p.1-10, 2019. https://doi.org/10.5423/PPJ.RW.08.2018.0168
Lecomte C, Alabouvette C, Edel-Hermann V, Robert F, Stein-berg, C. Biological control of ornamental plant diseases caused by Fusarium oxysporum: A review. Biological Con-trol, v.101, p.17-30, 2016. https://doi.org/10.1016/j.biocontrol.2016.06.004
Li C, Yang J, Li W, Sun J, Peng M. Direct root penetration and rhizome vascular colonization by Fusarium oxysporum f. sp. cubense are the key steps in the successful infection of Brazil Cavendish. Plant Disease, v.101, n.12, p.2073-2078, 2017. https://doi.org/10.1094/PDIS-04-17-0467-RE
Mckinney HH. Influence of soil, temperature and moisture on infection of wheat seedlings by Helmintho sporium sativum. Journal of Agricultural Research, Washington, v.26, p.195-217, 1923.
Pandey PK, Samanta R, Yadav, RNS. Inside the plant: ad-dressing bacterial endophytes in biotic stress alleviation. Ar-chives Microbiology, v.201, n.4, p. 415-429, 2019. https://doi.org/10. 1007/s00203-019-01642-y
Pereira VM, Borges CV, Brandão LP, Oliveira LS, Souza CPF, Gonçalves ZS, Silva SO, Santos-Serejo JA, Ferreira CF, Amorim EP, Ledo CAS. Genetic diversity between im-proved banana diploids using canonical variables and the Ward MLM Methods. Pesquisa Agropecuária Brasileira, v.47, n.10, p.1480-1488, 2012. https://doi.org/10.1590/S0100-204X2012001000010
Ploetz RC. Fusarium wilt of Banana. Review Phytopatology, v.105, p.1512-1521, 2015. https://doi.org/10.1094/PHYTO-04-15-0101-RVW
Rivera-Cruz MC, Narcia AT, Ballana GC, Kohler J, Coravaca F, Roldán A. Poultry manure and banana waste are effective biofertilizer carriers for promoting plant growth and soil sustainability in banana crops. Soil Biology and Biochemis-try, v.40, n.12, p.3092-3095, 2008. https://doi.org/10.1016/j.soilbio.2008.09.003
Roque RL, Amorim TB, Ferreira CF, Ledo CAS, Amorim EP. Desempenho agronômico de genótipos de bananeira no re-côncavo da Bahia. Revista Brasileira de Fruticultura, v.36 n.3, p.598-609, 2014. https://doi.org/10.1590/0100-2945-361/13
Silva ER. Exsudação radicular e sua utilização por rizobacté-rias. 2011. 23 p. Dissertação (mestrado em Gestão de recur-sos agroambientais). Faculdade de agricultura tropical e sub-tropical, Instituto Agronômico, Campinas.
Srivastava S, Sharma PK, Guru K. Nutraceuticals: a review. Journal of Chronotherapy and Drug Delivery, v.6, n.1, p.1-10, 2015.
Vieira LCS, Costa SN, Borges CV, Gonçalves ZS, Haddad F. Fusarium oxysporum f. sp. cubense biocontrol mediated by Bacillus spp. in Prata-Anã banana. Revista Brasileira de Ci-ências Agrárias, v.15, n.3, p.1-7, 2020. https://doi.org/10.5039/agraria.v15i3a8030
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 - Journal of Biotechnology and Biodiversity
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0 at http://creativecommons.org/licenses/by/4.0/) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Available at The Effect of Open Access, at http://opcit.eprints.org/oacitation-biblio.html).