Alves, S. A. O. et al. 3

J. Biotec. Biodivers. v. 2, N.2: pp. 1-6, May. 2011

In previous experiment, we observed that the supplementation with 0.17g.L NaH 2 PO 4 was indispensable for the obtaining normal plantlets (Data not shown).

Figure 2 - In vitro conversion of zygotic embryos to plantlets of oil palm from variety CI-2061. (A) Swelling embryos. (B) Curvature process of embryo. (C) Beginning of the root and aerial part of seedling and (D-F) Leave formation, (D-A.C) cotyledons and (D-R) Roots.

Embryos cultured on the ½ MS medium free of plant growth regulators (T1 treatment) presented the higher percentage of score 6 with 57.9%, being 20% to variety Cj-2141 and 37.93% to variety CI- 2061 (Figure 3). The varieties Cj-502 and Cj-594 obtained inferior development, they did not reach score 6 (Figure 3).

Figure 3 - Percentage of in vitro germination of embryos of the four varieties of interspecific hybrids of oil palm ( Elaeis oleifera x Elaeis guineensis ) on ½ MS medium (T1). Stages are represented by scores (Figure 1). Values obtained at 60 days of culture.

In the treatment T4, ½ MS medium supplemented with 0.5 mg.L BAP and NAA, it was obtained 51.03% of embryos with score 6, being 20% to variety Cj-2141 and 31% to variety CI-2061. The varieties Cj-502 and Cj-594 show inferior

development, they did not reach score 6 (Figure 4).

Figure 4 - Percentage of in vitro germination of embryos of the four varieties of interspecific hybrids of oil palm ( Elaeis oleifera x Elaeis guineensis ) on ½ MS medium + 0.5mg L NAA and BAP (T4). Stages are represented by scores (Figure 1). Values obtained at 60 days of culture.

In the T2 treatment, MS medium free of plant growth regulators, the percentage of score six varied from 0 to 37.93% (Figure 5). The variety that presented higher percentage of score 6 were the Cj-2141 with 37.93%, followed by Cj-2141 with 20% of score 6. However, the varieties Cj- 502 and Cj-494 did not reach score 6 (Figure 4).

Figure 5 - Percentage of in vitro germination of embryos of the four varieties of interspecific hybrids of oil palm ( Elaeis oleifera x Elaeis guineensis ) on MS medium (T2). Stages are represented by scores (Figure 1). Values obtained at 60 days of culture.

In the treatment T3, MS medium supplemented with BAP and NAA, the percentages of score 6 obtained were lower, nevertheless, the varieties